Multiplexed Fluorescence Resonance Energy Transfer Aptasensor between Upconversion Nanoparticles and Graphene Oxide for the Simultaneous Determination of Mycotoxins
Multiplexed Fluorescence Resonance Energy Transfer Aptasensor between Upconversion Nanoparticles and Graphene Oxide for the Simultaneous Determination of Mycotoxins
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DOI：
10.1021/ac301534w
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发表时间：
2012-07-17
影响因子：
7.4
通讯作者：
Zhang, Qian
中科院分区：
文献类型：
--
作者：
Wu, Shijia;Duan, Nuo;Zhang, Qian
我们提出了一种新的真菌毒素适体传感器，这是基于多重荧光共振能量转移（FRET）之间。上转换荧光纳米颗粒（UCNPs）作为供体，氧化石墨烯（GO）作为全部和有效的受体。合成了BaY 0. 78 F5：Y B-0. 2，Er-0. 02和BaY 0. 78 F5：Y B-0. 7，Tm-0. 02上转换纳米颗粒，并分别用固定化赭曲霉毒素A（OTA）-适体和伏马菌素B-1（FB 1）-适体进行功能化。基于适体的核碱基与GO的sp（2）原子之间的强π-π堆积效应，适体修饰的UCNP可以被带到靠近GO表面。BaY0.78F5：Yb-0.2，ET0.02和BaY0.78F5：Yb-0.2Tm-0.02的强上转换荧光都可以被GO完全猝灭，这是因为BaY0.78F5：Yb-0.2，ET0.02的荧光发射与BaY0.78F5：Yb-0.2Tm-0.02的吸收光谱有很好的重叠。走了相比之下，在OTA和FBI的存在下，适体更喜欢。结合到其相应的真菌毒素，这导致在形成的适配体的变化，因此，适配体修饰的UCNPs远离GO表面。研究结果表明，BaYF 5：Yb Er和BaYF 5：Yb Tin的荧光强度与OTA和FBI的浓度有关。因此，我们开发了一个敏感和简单的平台。用CLUCNP和GO作为FRET对同时检测OTA和FB 1。适体传感器提供了线性。方法的检出限为0.02 ng·mL（-1），检出限为0.1 ng·mL（-1）。应用该适体传感器对自然污染玉米样品中OTA和FB 1的检测结果与经典的ELISA方法一致。更重要的是，新的多重FRET。首次建立了基于多路复用的能量供体到整个能量受体。这项工作有望为FRET系统应用于各种目标开辟新的领域。
We presented a new aptasensor for mycotoxins, which was based on multiplexed fluorescence resonance energy transfer (FRET) between. multicolor upconversion fluorescent nanopartides (UCNPs) as donors and graphene oxide (GO) as the entire and effective acceptor. BaY0.78F5:Yb-0.2, Er-0.02 and BaY0.78F5:Yb-0.7, Tm-0.02 upconversion nanopartides were synthesized and functionalized, respectively, with immobilized ochratoxin A (OTA)-aptamers and fumonisin B-1 (FB1)-aptamers. On the basis of the strong pi-pi stacking effect between the nucleobases of the aptamers and the sp(2) atoms of GO, the aptamer modified-UCNPs can be brought in close proximity to the GO surface. The strong upconversion fluorescence both of BaY0.78F5:Yb-0.2, ET0.02 and BaY0.78F5:Yb-0.2 Tm-0.02 can be completely quenched by the GO, because of a good overlap between the fluorescence emission of multicolor UCNPs and the absorption spectrum of. GO. In contrast, in the presence of OTA and FBI, the aptamers preferred to. bind to their corresponding mycotoxins, which led to changes in the formation of aptamers, and therefore, aptamer modified-UCNPs were far away from the GO surface. Our study results showed that the fluorescence intensity of BaYF5:Yb Er and BaYF5:Yb Tin were related to the concentration Of OTA and FBI. We therefore developed a sensitive and simple platform for the. simultaneous detection of OTA and FB1 with multicolor UCNPs and GO as the FRET pair. The aptasensor provided a linear. range from 0.05 to 100 ng.mL(-1) for OTA and 0.1 to 500 ng.mL(-1) for FB1; the detection limit of OTA was 0.02 ng.mL(-1) and FB1 was 0.1 ng.mL(-1). As a practical application, the aptasensor was used to monitor OTA and FB1 level in naturally contaminated maize samples with the results consistent with that of a classic ELISA method. More importantly, the novel multiplexed FRET. was established for the first time based on multiplexed energy donors to the entire energy acceptor,. this work was expected to open up a new field of FRET system applications for various targets.