Inhibitory actions of several natural products on proliferation of rat vascular smooth muscle cells induced by Hsp60 from Chlamydia pneumoniae J138
Inhibitory actions of several natural products on proliferation of rat vascular smooth muscle cells induced by Hsp60 from Chlamydia pneumoniae J138
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DOI：
10.1021/jf0351164
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发表时间：
2004-10-06
影响因子：
6.1
通讯作者：
Matsuda, K
中科院分区：
文献类型：
--
作者：
Fukuoka, K;Sawabe, A;Matsuda, K
动脉粥样硬化是一种血管疾病，涉及炎症、整个图尼卡内膜中的血管腔变窄以及动脉壁弹性降低。已经发现，来自肺炎衣原体（一种与动脉粥样硬化病变相关的专性细菌病原体）的Hsp 60模拟人Hsp 60，从而引起免疫细胞对在其表面上表达内源性Hsp 60的应激内皮细胞的攻击。此外，C.已经显示，肺炎克雷伯氏菌促进血管平滑肌细胞（VSMC）的生长。为了探索可用于研究衣原体Hsp 60引起的信号转导的探针，我们测试了几种天然产物对Hsp 60诱导的大鼠动脉平滑肌细胞增殖的抑制作用。芝麻，香草醇，和反式阿魏酸表现出温和的抑制作用，热休克蛋白60诱导的细胞增殖; zerumbone，蛇麻烯，和carbylene有效地抑制它在低浓度的IC（50）值分别为529，122，和110 nM。结果表明，11元脂环有利于与Hsp 60诱导的VSMC增殖相关的受体相互作用。
Atherosclerosis is a vascular disorder involving inflammation, a narrowed vascular lumen in the entire tunica intima, and reduced elasticity of the arterial wall. It has been found that Hsp60 from Chlamydia pneumoniae, an obligate bacterial pathogen associated with atheroma lesions, mimics human Hsp60, thereby causing attacks by immune cells on stressed endothelial cells expressing endogenous Hsp60 on their surface. Furthermore, Hsp60 from C. pneumoniae has been shown to promote the growth of vascular smooth muscle cells (VSMCs). To explore probes that can be used for studying signal transduction elicited by the chlamydial Hsp60, we have tested several natural products for their inhibitory actions on the Hsp60-induced proliferation of rat arterial smooth muscle cells. Sesamol, vanillyl alcohol, and trans-ferulic acid exhibited moderate inhibitory actions on the Hsp60-induced cell proliferation; zerumbone, humulene, and caryophylene effectively inhibited it at low concentrations with IC(50) values of 529, 122, and 110 nM, respectively. The results indicated that the 11-membered alicyclic ring is favorable for interactions with receptors involved in the Hsp60-induced VSMC proliferation.