Induction of varicella zoster virus DNA replication in dissociated human trigeminal ganglia.
Induction of varicella zoster virus DNA replication in dissociated human trigeminal ganglia.
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DOI：
10.1007/s13365-016-0480-1
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发表时间：
2017-02
影响因子：
3.2
通讯作者：
Gilden D
中科院分区：
文献类型：
--
作者：
Cohrs RJ;Badani H;Baird NL;White TM;Sanford B;Gilden D
水痘带状疱疹病毒（VZV）是一种人类嗜神经性α疱疹病毒，在初次感染后潜伏并重新激活产生带状疱疹。为了研究VZV潜伏期和再激活，将死后24 h内切除的人三叉神经节机械解离，随机分布到六孔组织培养板中，用灭活神经生长因子（NGF）或磷酸肌肽3激酶（pi3激酶）途径的试剂孵育。在第5天，VZV DNA在对照和pi3激酶抑制剂处理的培养物中增加的程度相同，但在抗ngf处理的培养物中更丰富（p = 0.001）。总的来说，VZV DNA复制部分受pi3激酶不依赖的NGF通路调节。
Varicella zoster virus (VZV), a human neurotropic alphaherpesvirus, becomes latent after primary infection and reactivates to produce zoster. To study VZV latency and reactivation, human trigeminal ganglia removed within 24 h after death were mechanically dissociated, randomly distributed into six-well tissue culture plates and incubated with reagents to inactivate nerve growth factor (NGF) or phosphoinositide 3-kinase (PI3-kinase) pathways. At 5 days, VZV DNA increased in control and PI3-kinase inhibitor-treated cultures to the same extent, but was significantly more abundant in anti-NGF-treated cultures (p = 0.001). Overall, VZV DNA replication is regulated in part by an NGF pathway that is PI3-kinase-independent.