Electrophoresis of proteins and nucleic acids on acrylamide-agarose gels lacking covalent crosslinking.
Electrophoresis of proteins and nucleic acids on acrylamide-agarose gels lacking covalent crosslinking.
复制标题
蛋白质和核酸在缺乏共价交联的丙烯酰胺-琼脂糖凝胶上的电泳。
DOI：
10.1016/0003-2697(84)90672-9
复制
发表时间：
1984
影响因子：
2.9
通讯作者：
Barnes,LD
中科院分区：
文献类型：
--
作者：
Horowitz,PM;Lee,JC;Williams,GA;Williams,RF;Barnes,LD
本文报道了用亚甲基双丙烯酰胺制备无共价交联的丙烯酰胺琼脂糖凝胶。这些混合凝胶在85°C下熔化，因此，允许在电泳后定量分析氚标记的蛋白质。从混合凝胶中提取的氚标记核糖核酸的回收率比标准丙烯酰胺-亚甲基双丙烯酰胺凝胶高20 - 25%。电泳迁移率作为解离蛋白质和核糖核酸的分子量的函数的标准曲线进行了比较丙烯酰胺琼脂糖凝胶和丙烯酰胺-亚甲基双丙烯酰胺凝胶。
The preparation of acrylamide-agarose gels lacking covalent crosslinking with methylenebis-acrylamide is described. These hybrid gels melt at 85°C and, consequently, allow quantitative analysis of tritium-labeled protein after electrophoresis. Recovery of tritium-labeled ribonucleic acids extracted from hybrid gels is 20 to 25% greater than from standard acrylamide-methylenebisacrylamide gels. Standard curves of electrophoretic mobilities as a function of molecular weights of dissociated proteins and ribonucleic acids are compared for acrylamide-agarose gels and acrylamide-methylenebisacrylamide gels.