Advancing Biocapture Substrates via Chemical Lift-Off Lithography
Advancing Biocapture Substrates via Chemical Lift-Off Lithography
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DOI：
10.1021/acs.chemmater.7b01970
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发表时间：
2017-08-22
影响因子：
8.6
通讯作者：
Andrews, Anne M.
中科院分区：
文献类型：
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作者：
Cao, Huan H.;Nakatsuka, Nako;Andrews, Anne M.
创建用于高通量筛选或生物传感应用的小分子功能化平台需要在固体基质上精确放置探针，以及从多组分样品中捕获和分选目标的能力。在这里，化学剥离光刻被用来制作大面积、高保真的小分子探针图案。升空光刻使生物素链霉亲和素图案化识别成为可能，其特征尺寸从微米到30个边缘以下。通过提离的消减图案化促进了不同类型的分子的插入，从而多路并排放置小分子探针，使得结合伙伴从溶液中被定向到同源探针。模拟内源性神经递质的小分子被用提离光刻技术图案化，以捕获与膜相关的天然受体。我们表征了在具有不同末端官能团的Au上自组装的烷硫醇的图案化，以扩展服从于剥离光刻的分子库，从而实现了与这种简单而通用的图案化方法一起使用的广泛的官能化化学。
Creating small-molecule-functionalized platforms for high-throughput screening or biosensing applications requires precise placement of probes on solid substrates and the ability to capture and to sort targets from multicomponent samples. Here, chemical lift-off lithography was used to fabricate large-area, high-fidelity patterns of small-molecule probes. Liftoff lithography enables biotin streptavidin patterned recognition with feature sizes ranging from micrometers to below 30 rim. Subtractive patterning via lift-off facilitated insertion of a different type of molecule and, thus, multiplexed side-by-side placement of small-molecule probes such that binding partners were directed to cognate probes from solution. Small molecules mimicking endogenous neurotransmitters were patterned using lift-off lithography to capture native membrane-associated receptors. We characterized patterning of alkanethiols that self-assemble on Au having different terminal functional groups to expand the library of molecules amenable to lift-off lithography enabling a wide range of functionalization chemistries for use with this simple and versatile patterning method.