Effects of unclipping and converting enzyme inhibition on bilateral renal function in Goldblatt hypertensive rats.
Effects of unclipping and converting enzyme inhibition on bilateral renal function in Goldblatt hypertensive rats.
复制标题
解夹和转换酶抑制对戈德布拉特高血压大鼠双侧肾功能的影响。
DOI：
10.1038/ki.1983.100
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发表时间：
1983
影响因子：
19.6
通讯作者：
Navar,LG
中科院分区：
文献类型：
--
作者：
Huang,WC;Navar,LG
方法 通过将 0.25 毫米银夹子放置在 80 至 110 g Sprague-Dawley 雄性大鼠（Charles River Breeding Laboratories，Wilmington，Maryland）的左肾动脉上来制备两肾一夹 Goldblatt 高血压大鼠。实验前3至4周，在戊巴比妥麻醉(5.0mg1100g，腹腔注射)下进行剪除程序。所有大鼠均用商业大鼠饲料（Wayne Lab Blox，芝加哥，伊利诺伊州）喂养，每克饲料含有 0.5 mEq 钠，并随意给予自来水。急性实验在剪毛后第 4 周内进行，此时高血压大鼠体重为 200 至 290 g。用戊巴比妥钠(5.0mg1100g，腹膜内注射)麻醉大鼠。手术准备与之前描述的类似[21。简而言之，准备大鼠在加热台上进行清除实验，并通过监测直肠温度的恒温系统将体温维持在37℃。气管插管并插入颈外静脉导管以输注溶液和药物。使用 Statham P23Dc 传感器（Gould-Statham Instruments Inc.，Hato Rey，波多黎各）和 P7 Grass 测谎仪（Grass Instrument Co.，Quincy，Massachusetts）对左股动脉插管进行血液采样和血压测量。通过横向方法，暴露左肾（被夹住的）并将其放置在杯子（Lucite®）中。向左输尿管和膀胱插入导管，连续从两个肾脏收集尿液。仔细解剖左肾动脉银夹周围的纤维组织，不损伤肾动脉或引起出血。这种方法使得夹子可以在实验后期轻松移除。在手术过程中，以0.02 mi/mm的速率给大鼠输注等渗盐水。手术完成后，给予聚果聚糖（mutest®，10 gIdl，Laevosan-Gesellschaft，林茨，奥地利）和对氨基马尿酸盐（PAH，2 g/dl，Merck Sharp and Dohme，West Point，宾夕法尼亚州）混合溶液的启动剂量，然后以 0.01 ml/min 的速率持续输注。为了保持总体积输注速率恒定，将盐水输注速率降低至0.01ml/min。让大鼠在 30 毫米内达到稳定状态，然后使用 30 毫米的清除期收集尿液样本。在每个时期的中点采集血样。分离血浆并将血细胞返回给动物。
Methods Two-kidney, one clip Goldblatt hypertensive rats were pre-pared by placing a 0.25-mm silver clip on the left renal artery of 80 to 110 g Sprague-Dawley male rats (Charles River Breeding Laboratories, Wilmington, Maryland). The clipping procedure was performed under pentobarbitol anesthesia (5.0 mgIlOO g, ip) 3 to 4 weeks prior to the experiments. All rats were fed with commercial rat chow (Wayne Lab Blox, Chicago, Illinois) containing 0.5 mEq sodium per gram of chow and were given tap water ad libitum.The acute experiments were carried out within week 4 postclipping at which time the hypertensive rats weighed 200 to 290 g. Rats were anesthetized with sodium pentobarbitol (5.0 mgIlOO g, ip). The surgical preparations were similar to those described previously [21. Briefly, rats were prepared for clearance experiments on a heated table and body temperature was maintained at 37 C by a thermostatic system monitoring the rectal temperature. The trachea was cannulated and an external jugular vein catheter was inserted for infusion of solutions and drugs. The left femoral artery was catheterized for blood sampling and measurement of blood pressure using a Statham P23Dc transducer (Gould-Statham Instruments Inc., Hato Rey, Puerto Rico) and a P7 Grass polygraph (Grass Instrument Co., Quincy, Massachusetts). Through a lateral approach, the left (clipped) kidney was exposed and placed in a cup (Lucite®). The left ureter and the urinary bladder were catheterized for sequential collection of urine from both kidneys. The fibrous tissue surrounding the silver clip on the left renal artery was carefully dissected free without damaging the renal artery or causing bleeding. This approach allowed the clip to be removed easily later in the experiment. During the surgical operation, rats were infused with isotonic saline at a rate of 0.02 mi/mm. Upon completing the surgery, a priming dose of a mixed solution of polyfructosan (mutest®, 10 gIdl, Laevosan-Gesellschaft, Linz, Austria) and para-aminohip-purate (PAH, 2 g/dl, Merck Sharp and Dohme, West Point, Pennsylvania) was administered and followed by a sustaining infusion at a rate of 0.01 ml/min. To maintain the total volume infusion rate constant, the saline infusion rate was decreased to 0.01 ml/min. Rats were allowed to reach a steady state for 30 mm, and then urine samples were collected using 30-mm clearance periods. Blood samples were taken at the midpoint of each period. Plasma was separated and blood cells were returned to the animals.