Antibiotic Resistome and Its Association with Bacterial Communities during Sewage Sludge Composting
Antibiotic Resistome and Its Association with Bacterial Communities during Sewage Sludge Composting
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DOI：
10.1021/acs.est.5b01012
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发表时间：
2015-06-16
影响因子：
11.4
通讯作者：
Zhu, Yong-Guan
中科院分区：
文献类型：
--
作者：
Su, Jian-Qiang;Wei, Bei;Zhu, Yong-Guan
堆肥被广泛用于城市污水污泥的资源化，以改善土壤性质，这是一个潜在的途径，传播抗生素耐药细菌和基因到土壤中。然而，抗生素耐药基因（ARGs）的动态和潜在的机制，在污泥堆肥没有得到充分的探讨。在这里，我们使用高通量定量PCR和16S rRNA基因为基础的Illumina测序，调查在实验室规模的容器堆肥的污水污泥的ARGs和细菌群落的动态。共检测到156个独特的ARG和移动的遗传元件（MGE）编码几乎所有主要类别的抗生素耐药性。ARGs检测到显着增加的丰度和多样性，和独特的模式，并在堆肥过程中丰富。在堆肥过程中观察到细菌群落结构和组成的显着变化，放线菌是在堆肥后期的优势门。放线菌的大比例可以部分解释堆肥过程中ARGs的增加。ARGs的模式与细菌群落结构显着相关，表明ARGs的动态在堆肥过程中的细菌系统发育组成的强烈影响。这些结果表明，直接施用污泥堆肥的领域可能会导致大量的ARGs在土壤中的传播。
Composting is widely used for recycling of urban sewage sludge to improve soil properties, which represents a potential pathway of spreading antibiotic resistant bacteria and genes to soils. However, the dynamics of antibiotic resistance genes (ARGs) and the underlying mechanisms during sewage sludge composting were not fully explored. Here, we used high-throughput quantitative PCR and 16S rRNA gene based illumina sequencing to investigate the dynamics of ARGs and bacterial communities during a lab-scale in-vessel composting of sewage sludge. A total of 156 unique ARGs and mobile genetic elements (MGEs) were detected encoding resistance to almost all major classes of antibiotics. ARGs were detected with significantly increased abundance and diversity, and distinct patterns, and were enriched during composting. Marked shifts in bacterial community structures and compositions were observed during composting, with Actinobacteria being the dominant phylum at the late phase of composting. The large proportion of Actinobacteria May partially explain the increase of ARGs during composting. ARGs patterns were significantly correlated with bacterial community structures, suggesting that the dynamic of ARGs was strongly affected by bacterial phylogenetic compositions during composting. These results imply that direct application of sewage sludge compost on field may lead to the spread of abundant ARGs in soils.