Bambi is coexpressed with Bmp-4 during mouse embryogenesis
Bambi is coexpressed with Bmp-4 during mouse embryogenesis
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DOI：
10.1016/s0925-4773(00)00524-4
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发表时间：
2001-02-01
影响因子：
2.6
通讯作者：
Rüther, U
中科院分区：
文献类型：
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作者：
Grotewold, L;Plum, M;Rüther, U
TGF-β超家族成员的信号传导受到精心设计的调控网络的严格控制（最近的综述见Trends Genet. 15（1999）3; Genes Dev. 14（2000）627）。最近，已显示跨膜蛋白BAMBI（BMP和激活素膜结合抑制剂）通过抑制Tgf-β I型受体活化来干扰BMP和激活素样信号传导（Nature 401（1999）480）。与其他Bmp拮抗剂如头蛋白（Cell 86（1996）599）或脊索蛋白（Cell 86（1996）589）形成鲜明对比，BAMBI在早期非洲爪蟾胚胎发生期间与Bmp-4严格共表达。根据它们共享的复杂空间表达模式和它们参与相同的生物信号传导途径的基因分组被称为同表达组。这一概念有助于对功能未知的组成员的角色进行分析。显然，只有少数基因被组织在同表达组中，到目前为止，它们主要在酵母和非洲爪蟾中被描述（综述参见Nature 402（1999）483）。在青蛙中，BAMBI是Bmp-4同向表达组的成员（Nature 401（1999）480）。我们确定了两个鼠同源BAMBI其中之一，命名为Bambi-psi，是一个假基因。我们发现Bambi的时空表达模式与Bmp-4在小鼠胚胎发育过程中的时空表达模式密切匹配，此外，我们还发现Bambi的表达在小鼠胚胎成纤维细胞中由Bmp-4诱导。因此，我们提供了第一个证据，在哺乳动物中存在一个进化上保守的BMP-4同向表达组。(C)2001 Elsevier Science Inland Ltd.保留所有权利。
Signaling of TGF-beta superfamily members is tightly controlled by an elaborate network of regulators (for recent review see Trends Genet. 15 (1999) 3; Genes Dev. 14 (2000) 627). Recently, the transmembrane protein BAMBI (BMP and activin membrane-bound inhibitor) has been shown to interfere with Bmp and activin-like signaling by inhibiting Tgf-beta type I receptor activation (Nature 401 (1999) 480). In striking contrast to other Bmp antagonists like noggin (Cell 86 (1996) 599) or chordin (Cell 86 (1996) 589), BAMBI is strictly coexpressed with Bmp-4 during early Xenopus embryogenesis. The grouping of genes according to their shared complex spatial expression pattern and their involvement in the same biological signaling pathway has been referred to as synexpression group. This concept facilitates prognoses about the roles of a group member with unknown function. Apparently, only a minority of genes is organized in synexpression groups and up to now they have mainly been described in yeast and Xenopus (for review see Nature 402 (1999) 483). In the frog, BAMBI is a member of the Bmp-4 synexpression group (Nature 401 (1999) 480). We identified two murine homologues of BAMBI one of which, named Bambi-psi, is a pseudogene. We show that the spatiotemporal expression pattern of Bambi closely matches that of Bmp-4 during mouse embryonic development, Moreover, we show that Bambi expression is induced in mouse embryonic fibroblasts by Bmp-4. Hence, we provide first evidence for the existence of an evolutionarily conserved Bmp-4 synexpression group in mammals. (C) 2001 Elsevier Science Inland Ltd. All rights reserved.