MALDI-TOF-MS analysis of protein and DNA
MALDI-TOF-MS analysis of protein and DNA
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DOI：
10.1177/107385840100700104
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发表时间：
2001-02-01
期刊：
影响因子：
5.6
通讯作者：
Humeny, A
中科院分区：
文献类型：
--
作者：
Bonk, T;Humeny, A
基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）已成为生命科学各个领域中一种强大而广泛的分析工具。宽质量范围（1-300 kDa）、高准确度和灵敏度使其成为分析包括蛋白质、核酸和碳水化合物在内的所有种类生物分子的上级方法。结合2D-凝胶电泳，MALDI-TOF-MS特别适用于通过质量指纹或微测序鉴定蛋白质点。此外，该方法允许详细分析翻译后蛋白质修饰。最近，该方法也成功地应用于DNA测序以及突变筛查。因此，单核苷酸多态性的高通量基因分型有可能成为实验室和临床应用的常规方法。
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has become a powerful and widespread analytical tool in all fields of life science. The wide mass range (1-300 kDa), high accuracy, and sensitivity make it a superior method for analysis of all kinds of biomolecules including proteins, nucleic acids, and carbohydrates. In combination with 2D-gelelectrophoresis, MALDI-TOF-MS is particularly suitable for the identification of protein spots via mass fingerprint or microsequencing. Furthermore, the method allows a detailed analysis of posttranslational protein modifications. Recently, the method was also successfully applied to DNA sequencing as well as screening for mutations. Thus, high-throughput genotyping of single nucleotide polymorphisms has the potential to become a routine method for both laboratory and clinical applications.