Nicotiana benthamiana: Its History and Future as a Model for Plant-Pathogen Interactions.
Nicotiana benthamiana: Its History and Future as a Model for Plant-Pathogen Interactions.
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DOI：
10.1094/mpmi-00-00-1015-rev.testissue
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
M. Goodin;D. Zaitlin;R. Naidu;S. Lommel
中科院分区：
文献类型：
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作者：
M. Goodin;D. Zaitlin;R. Naidu;S. Lommel
本氏烟草（Nicotianabenthamiana）是植物病毒学中应用最广泛的实验寄主，主要是由于大量不同的植物病毒可以成功地侵染它。本塞姆氏菌对多种其它植物病原体（例如细菌、卵菌、真菌等）敏感，使得该物种成为宿主-病原体研究的基石，特别是在先天免疫和防御信号传导的背景下。此外，由于它可以高效地进行遗传转化和再生，并且适合病毒诱导的基因沉默或瞬时蛋白质表达的简单方法，因此N.本塞姆氏菌在植物生物学中，特别是在需要蛋白定位、相互作用或用于蛋白表达和纯化的基于植物的系统的研究中迅速流行。巧合的是，尽管是一个不可或缺的研究模式，但对N的起源，遗传变异或生态学知之甚少。目前研究界使用的Benthamiana种质。除了广告- dressing这些后者的主题，这篇评论的目的是提供有关的工具和试剂，可用于支持在N的研究来源的信息。本萨米亚纳最后，我们提出N. benthamiana很有可能成为一个首要的植物细胞生物学模型，特别是对于病毒学界来说，他们作为一个群体是第一个认识到这种独特的澳大利亚本土植物的潜力的。
Nicotiana benthamiana is the most widely used experimental host in plant virology, due mainly to the large number of diverse plant viruses that can successfully infect it. Addi- tionally, N. benthamiana is susceptible to a wide variety of other plant-pathogenic agents (such as bacteria, oomycetes, fungi, and so on), making this species a cornerstone of host-pathogen research, particularly in the context of innate immunity and defense signaling. Moreover, because it can be genetically transformed and regenerated with good efficiency and is amenable to facile methods for virus- induced gene silencing or transient protein expression, N. benthamiana is rapidly gaining popularity in plant biology, particularly in studies requiring protein localization, inter- action, or plant-based systems for protein expression and purification. Paradoxically, despite being an indispensable research model, little is known about the origins, genetic variation, or ecology of the N. benthamiana accessions cur- rently used by the research community. In addition to ad- dressing these latter topics, the purpose of this review is to provide information regarding sources for tools and reagents that can be used to support research in N. benthamiana. Finally, we propose that N. benthamiana is well situated to become a premier plant cell biology model, particularly for the virology community, who as a group were the first to recognize the potential of this unique Australian native.