LARVICIDAL ACTIVITY AND INFLUENCE OF BACILLUS THURINGIENSIS VAR. ISRAELENSIS ON AEDES ALBOPICTUS OVIPOSITION IN OVITRAPS DURING A TWO-WEEK CHECK INTERVAL PROTOCOL
LARVICIDAL ACTIVITY AND INFLUENCE OF BACILLUS THURINGIENSIS VAR. ISRAELENSIS ON AEDES ALBOPICTUS OVIPOSITION IN OVITRAPS DURING A TWO-WEEK CHECK INTERVAL PROTOCOL
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DOI：
10.2987/08-5852.1
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发表时间：
2009-06-01
影响因子：
1
通讯作者：
Bellini, Romeo
中科院分区：
文献类型：
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作者：
Carrieri, Marco;Masetti, Antonio;Bellini, Romeo
苏云金芽孢杆菌变种的毒力持效性研究。通过实验室和现场试验对白纹伊蚊（Bti）进行了评价，以建立一种新的白纹伊蚊监测方法。在实验室条件下，以24 μ g/L Bti处理的尖音库蚊幼虫在第4、8、12和16天的死亡率为指标，比较了5种Bti制剂的残留毒力。结果显示所有测试制剂的良好性能（对于所有制剂，在第14天>97%的死亡率），但只有浓度为1 ml/升的VectobacTM 12 AS在2周内显示100%的所有功效。通过田间试验，研究了Bti油对诱卵器检查间隔时间的影响及对妊娠白纹伊蚊产卵反应的影响。白纹伊蚊雌性使用三种不同的诱卵器处理：诱卵器与自来水每周检查一次，诱卵器与自来水每2周检查一次;诱卵器与Bti（Vectobac 12 AS，剂量为1 ml/L）每2周检查一次。我们的研究表明，在诱卵器中，1%的Bti溶液的毒性作用至少维持14天，死亡率为100%，降雨似乎没有负面影响Bti的残留作用。因此，用Bti诱蚊产卵器完成幼虫发育周期的可能性似乎很低。产卵活性指数表明，Bti对Ae.白纹伊蚊占17.4%。
Toxicity persistence of Bacillus thuringiensis var. israelensis (Bti) was evaluated in laboratory and field trials to develop a new protocol For Aedes albopictus monitoring. In the laboratory, the residual toxicity of 5 Bti formulations wits compared using the Percentage mortality at days 4, 8, 12, and 16 of Culex pipiens larvae exposed for 24 It to Bti treatments. The results showed a good performance of all tested formulations (>97% mortality at day 14, for all the formulations), but only Vectobac (TM) 12AS at the concentration of 1 ml/liter showed all efficacy of 100% for 2 wk. A field study was designed to test the effect of Bti oil the ovitrap check interval or influence of ovipositional response of gravid Ae. albopictus females. Three different ovitrap treatments were used: ovitraps with tap water checked weekly, ovitraps with tap water checked every 2 wk; ovitraps with Bti (Vectobac 12AS, dose of 1 ml/liter) checked every 2 wk. Our study demonstrated that in the ovitrap, the toxic action of a 1% Solution of Bti was maintained for at least 14 days with mortality of 100% and that rainfall did not seem to negatively influence the residual action of Bti. Therefore file probability that the larvae may complete the developmental cycle in ovitraps with Bti seems to be very low. The oviposition activity index showed that Bti enhances the oviposition rate of Ae. albopictus by 17.4%.