Network of tangential pathways for neuronal migration in adult mammalian brain
Network of tangential pathways for neuronal migration in adult mammalian brain
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DOI：
10.1073/pnas.93.25.14895
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发表时间：
1996-12-10
影响因子：
11.1
通讯作者：
AlvarezBuylla, A
中科院分区：
文献类型：
--
作者：
Doetsch, F;AlvarezBuylla, A
成年哺乳动物的大脑细胞在整个侧脑室侧壁的室下区（SVZ）继续增殖。在这里，我们通过对成年小鼠侧脑室侧壁的整个坐骨解剖发现，SVZ被组织成一个广泛的神经元前体链网络，这些链对多涎化形式的NCAM（一种存在于可塑性部位的分子）和TuJ1（一种早期神经元标记物）具有免疫阳性。大多数链沿着吻侧轴定向，许多链加入吻侧迁移流，最终在嗅球处终止。通过局部显微注射DiI和移植携带神经元特异性报告基因的SVZ细胞，我们证明了起源于SVZ的不同背侧侧水平的细胞向背侧迁移并到达嗅球，在那里它们分化成神经元。我们的研究结果揭示了神经元前体在成年哺乳动物大脑侧脑室侧壁的切向链迁移的广泛通路网络。
Cells in the brains of adult mammals continue to proliferate in the subventricular zone (SVZ) throughout the lateral wall of the lateral ventricle, Here we show, using whole mount dissections of this wall from adult mice, that the SVZ is organized as an extensive network of chains of neuronal precursors, These chains are immunopositive to the polysialylated form of NCAM, a molecule present at sites of plasticity, and TuJ1, an early neuronal marker. The majority of the chains are oriented along the rostrocaudal axis and many join the rostral migratory stream that terminates in the olfactory bulb. Using focal microinjections of DiI and transplantation of SVZ cells carrying a neuron-specific reporter gene, we demonstrate that cells originating at different rostrocaudal levels of the SVZ migrate rostrally and reach the olfactory bulb where they differentiate into neurons, Our results reveal an extensive network of pathways for the tangential chain migration of neuronal precursors throughout the lateral wall of the lateral ventricle in the adult mammalian brain.