Relative amounts of sialic acid and fucose of amniotic fluid glycoconjugates in relation to pregnancy age
Relative amounts of sialic acid and fucose of amniotic fluid glycoconjugates in relation to pregnancy age
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DOI：
10.1007/s10719-005-4437-5
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发表时间：
2005-11-01
影响因子：
3
通讯作者：
Katnik-Prastowska, I
中科院分区：
文献类型：
--
作者：
Orczyk-Pawilowicz, M;Florianski, J;Katnik-Prastowska, I
目前关于羊水糖偶联物的糖链结构和作用的知识是零散的，主要集中在单个糖蛋白上。问题是羊水糖偶联物的一般糖基化模式是否会随着正常妊娠的进展而改变。在目前的工作中，我们描述了与妊娠年龄有关的羊水糖偶联物亚末端低聚糖结构的各种异常连接连接的唾液酸和岩藻糖相对量的动态、定量的变化。用凝集素斑点法对正常妊娠羊水进行分析：中期妊娠(14~19周)、晚期妊娠(29~37周)、围产期(38~40周)、足月分娩(39~41周)和过期妊娠(41~43周)。与妊娠中期相比，妊娠晚期羊水糖偶联物中以α2-6连接的唾液酸(p<；0.00002)和α1-6最内层岩藻糖(p<；0.000001)终止的糖链的相对含量较高。相反，他们显示岩藻糖连接的α1-3的相对含量较低(p<；0.02)。在围产期，α2-6连接唾液酸的相对量增加(p<；0.03)，然后在分娩期间下降(p<；0.02)，降至“晚期妊娠”组的水平。在过期妊娠中，研究的所有参数都增加了。羊水糖偶联物中的唾液糖基和岩藻糖苷可能在胎儿的生长和组织重塑中起关键作用，也可能反映胎儿的成熟。此外，糖位表达的测定可能有助于产前诊断，作为母子幸福的预测因素。
The present knowledge concerning the glycan structures and role of glycoconjugates derived from amniotic fluid is fragmentary and mainly focuses on the individual glycoproteins. The question has arisen as whether the general glycosylation pattern of amniotic fluid glycoconjugates can change with the progression of a normal pregnancy. In the present work we have described the dynamic, quantitative alterations in relative amounts of sialic acid and fucose linked by a variety of anomeric linkages to subterminal oligosaccharide structures of amniotic fluid glycoconjugates in relation to pregnancy age. The analysis was performed in the following groups of amniotic fluids derived from normal pregnancy by lectin dotting method: "2nd trimester" (14-19 weeks), "3rd trimester" (29-37 weeks), "perinatal period" (38-40 weeks) , "delivery at term" (39-41 weeks) and "post date pregnancy" (41-43 weeks). In the "3rd trimester" the amniotic fluid glycoconjugates contained higher relative amounts of glycans terminated by alpha 2-6-linked sialic acid (p < 0.00002) and by alpha 1-6 innermost fucose (p < 0.000001) than those in the 2nd trimester. In contrast, they showed the lower relative amount of fucose linked alpha 1-3 (p < 0.02). At the perinatal period the relative amount of alpha 2-6-linked sialic acid increased (p < 0.03), and it then decreased during delivery (p < 0.02) to the level found in the "3rd trimester" group. In the post date pregnancy all parameters studied increased. The sialyl- and fucosyl-glycotopes of the amniotic fluid glycoconjugates may play an critical role in growth and tissue remodeling of the foetus, as well as may might reflect maturation of a foetus. Additionally, a determination of the glycotope expressions might be helpful in prenatal diagnosis as predictor factors for well being of mother and child.