Multiple fucosyltransferases and their carbohydrate ligands are involved in spermatogenic cell-Sertoli cell adhesion in vitro in rats.
Multiple fucosyltransferases and their carbohydrate ligands are involved in spermatogenic cell-Sertoli cell adhesion in vitro in rats.
复制标题
多种岩藻糖基转移酶及其碳水化合物配体参与大鼠体外生精细胞-支持细胞的粘附。
DOI：
10.1095/biolreprod56.5.1268
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发表时间：
1997
影响因子：
3.6
通讯作者：
Millette,CF
中科院分区：
文献类型：
--
作者：
Raychoudhury,SS;Millette,CF
我们在大鼠精系上皮细胞上发现了多个聚焦转移酶（α[1- 2]-, α[1-3]-, α[1-4]-FTs），通过聚焦分别掺入苯基-β-半乳糖苷（Ph-β- d - gal）， 2 ' -聚焦乳糖（2 ' - fl）和乳酸- n -fucopentaose- l （LNF I）。现在，利用荧光激光扫描细胞术，我们报道了多种FTs与体外生殖细胞-支持细胞粘附有关。从19 ~ 21日龄的CD大鼠中分离支持细胞，培养6 ~ 10天。将成年大鼠睾丸经酶分散得到混合生殖细胞，培养过夜后用荧光指示剂钙黄蛋白的10 μM乙酰氧基甲酯衍生物标记。用570型激光细胞仪测定在存在或不存在各种低分子量受体进行聚焦的情况下，支持细胞单层上标记的贴壁生殖细胞数量。标记的生殖细胞与Sertoli细胞单层共孵育，在GDP-聚焦物、udp -半乳糖、Ph-β- d -gal、2 ' - fl、LNF I、Lewis-X和3 ' -sialyl-Lewis-X寡糖的存在下，与未处理的对照组或与不作为聚焦受体的纤维二糖、蜜二糖和α-Dmannopyranose孵育的对照样品相比，生殖细胞结合显著减少。我们的研究结果表明，多种FTs及其凝集素/选择素配体参与介导生殖细胞-支持细胞粘附形成内聚上皮，从而帮助生殖细胞在精原上皮内的腔内易位。
We have identified multiple fucosyltransferases (FTs) (α[1- 2]-, α[1-3]-, α[1-4]-FTs) on cells of the rat seminiferous epithelium as demonstrated by fucose incorporation into phenyl-β-Dgalactoside (Ph-β-D-Gal), 2’-fucosyllactose (2’-FL), and lacto-N-fucopentaose- l (LNF I), respectively. Now, using fluorescence laser scanning cytometry, we report that multiple FTs are implicated in germ cell-Sertoli cell adhesion in vitro. Sertoli cells were isolated from 19- to 21-day-old CD rats and cultured for 6–10 days. Mixed germ cells were obtained by enzymatic dispersion of adult rat testis and cultured overnight before labeling with 10 μM acetoxymethyl ester derivative of the fluorescent indicator, calcein. The adherent cell analysis and sorting 570 interactive laser cytometer was used to determine the number of labeled adherent germ cells on Sertoli cell monolayers in the presence or absence of a variety of low molecular weight acceptors for fucose. Coincubation of labeled germ cells with Sertoli cell monolayers in the presence of GDP-fucose, UDP-galactose, Ph-β-D-Gal, 2’-FL, LNF I, and Lewis-X and 3’-sialyl-Lewis-X oligosaccharides resulted in significant reduction of germ cell binding when compared to that of the untreated controls or of control samples incubated with cellobiose, melibiose, and α-Dmannopyranose, which do not serve as fucose acceptors. Our results suggest that multiple FTs and their lectin/selectin ligands are involved in mediating germ cell-Sertoli cell adhesion to form a cohesive epithelium and thus aid germ cell adluminal translocation within the seminiferous epithelium.