Disordered expression of HOX genes in human non-small cell lung cancer.
Disordered expression of HOX genes in human non-small cell lung cancer.
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DOI：
10.3892/or.15.4.797
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发表时间：
2006-04
期刊：
影响因子：
4.2
通讯作者：
Motoki Abe;J. Hamada;Osamu Takahashi;Yoko Takahashi;M. Tada;M. Miyamoto;T. Morikawa;S. Kondo;T. Moriuchi
中科院分区：
文献类型：
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作者：
Motoki Abe;J. Hamada;Osamu Takahashi;Yoko Takahashi;M. Tada;M. Miyamoto;T. Morikawa;S. Kondo;T. Moriuchi
我们假设癌症中的组织结构紊乱是由于细胞以时空不适当的方式执行个体发育过程中设计的程序造成的。 HOX基因被称为胚胎形态发生的主调控因子，其编码的转录因子调控下游基因的转录，从而实现机体规划的程序。在本研究中，我们通过基于实时RT-PCR方法的综合分析系统定量了41个人类非小细胞肺癌（non-SCLC）和非癌性肺组织中39个HOX基因的表达水平。我们发现鳞状细胞癌组织中HOXA1、A5、A10和C6（以及腺癌组织中HOXA5和A10）的表达水平显着高于非癌组织。比较腺癌和鳞癌组织中HOX基因表达量发现,鳞癌组织中HOXA1、D9、D10、D11的表达量高于腺癌组织。免疫组织化学分析显示，HOXA5和A10蛋白定位于腺癌和鳞状细胞癌组织中肿瘤细胞的细胞质中。这些结果表明HOX基因表达的紊乱模式不仅与非SCLC的发展有关，而且还与腺癌和鳞状细胞癌等组织学异常多样性有关。
We hypothesized that the disordered tissue architecture in cancer results from the cells executing the program designed during ontogeny in a spatio-temporally inappropriate manner. HOX genes are known as master regulators of embryonic morphogenesis, and encode transcription factors which regulate the transcription of the downstream genes to realize the program of body plan. In this study, we quantified the expression levels of 39 HOX genes in 41 human non-small cell lung cancer (non-SCLC) and non-cancerous lung tissues by a comprehensive analysis system based on the real-time RT-PCR method. We found that the expression levels of HOXA1, A5, A10 and C6 in squamous cell carcinoma tissues (and HOXA5 and A10 in adenocarcinoma tissues) were significantly higher than those in the non-cancerous tissues. Comparison of HOX gene expressions between adenocarcinoma and squamous cell carcinoma tissues showed higher expressions of HOXA1, D9, D10 and D11 in squamous cell carcinoma tissues than in adenocarcinoma tissues. Immunohistochemical analysis revealed that HOXA5 and A10 proteins were localized in the cytoplasm of tumor cells in both adenocarcinoma and squamous cell carcinoma tissues. These results suggest that the disordered patterns of HOX gene expressions were involved not only in the development of non-SCLC but also in the histologically aberrant diversity such as adenocarcinoma and squamous cell carcinoma.