The degradation of subalpine meadows significantly changed the soil microbiome
The degradation of subalpine meadows significantly changed the soil microbiome
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DOI：
10.1016/j.agee.2023.108470
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发表时间：
2023-06
期刊：
影响因子：
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通讯作者：
Kaifang Liu;Ting Li;X. Duan;Song Zhang;Meiping Chen;Haiyan Hou;Zelin Wang;Anlan Yu;
中科院分区：
文献类型：
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作者：
Kaifang Liu;Ting Li;X. Duan;Song Zhang;Meiping Chen;Haiyan Hou;Zelin Wang;Anlan Yu;
草原退化严重影响地球的健康，从而削弱了草原提供多种生态系统服务的能力。土壤微生物在退化土地的演替过程中被认为是游戏规则的改变者，因此，确定它们对草地退化的响应对于退化草地的恢复至关重要。然而，土壤微生物对草地退化的响应还远未得到很好的理解，特别是对低纬度草甸。因此，本研究在中国南方的一个自然退化亚高山草甸和两个人工退化亚高山草甸上进行，以确定土壤微生物对低纬度亚高山草甸退化的响应。在每个站点，土壤收集非退化，中度退化，严重退化亚高山草甸。采用实时PCR和扩增子NovaSeq测序技术分析土壤微生物丰度和多样性。我们发现，亚高山草甸的退化显着降低了几乎所有的土壤养分的浓度，并大大改变了土壤pH值和质地。我们还发现，土壤微生物表现出几乎一致的反应，自然和人工亚高山草甸退化。具体而言，降解不影响土壤微生物丰度，但显着改变微生物群落概况。大多数植物生长促进微生物类群的相对丰度显着降低亚高山草甸退化，而微生物类群，对植物健康不利的表现出相反的趋势。土壤微生物丰富度也显着降低了亚高山草甸的严重退化。此外，亚高山草甸的严重退化显着降低了微生物共生网络的复杂性和稳定性。上述土壤微生物组的变化也显示出与植物生物量的显著相关性。总之，土壤微生物的变化是影响亚高山草甸退化的关键因素，调控土壤微生物是一种有前途的恢复策略。
Grassland degradation seriously affects the health of our planet, thereby weakening the ability of grasslands to provide multiple ecosystem services. Soil microbes have been recognized as game changers in the succession of degraded lands; therefore, the determination of their responses to grassland degradation is crucial for restoring degraded grasslands. However, soil microbial responses to grassland degradation are still far from being well understood, especially for low-latitude meadows. Hence, this study was performed in one natural and two artificial degraded subalpine meadows in Southern China to identify the soil microbial responses to the degradation of low-latitude subalpine meadows. At each site, soils were collected from nondegraded, moderately degraded, and heavily degraded subalpine meadows. Soil microbial abundance and diversity were analyzedviareal-time PCR and amplicon NovaSeq sequencing, respectively. We found that the degradation of subalpine meadows significantly decreased the concentrations of almost all of the soil nutrients and considerably changed soil pH and texture. We also found that soil microbes demonstrated almost consistent responses to the degradation of the natural and artificial subalpine meadows. Specifically, degradation did not affect soil microbial abundance but significantly changed microbial community profiles. The relative abundance of most plant growth-promoting microbial taxa significantly decreased with subalpine meadow degradation, whereas the microbial taxa that were adverse to plant health exhibited a reverse trend. Soil microbial richness was also significantly decreased by the heavy degradation of the subalpine meadows. Additionally, the heavy degradation of the subalpine meadows significantly decreased the complexity and stability of the microbial co-occurrence network. The aforementioned soil microbiome changes also showed the significant correlations with plant biomass. Collectively, the changes in the soil microbiome can be critical factors affecting the degradation of subalpine meadows, and the regulation of soil microbiome is a promising restoration strategy.