pH dependence of the redox potential of Pseudomonas aeruginosa cytochrome c-551.
pH dependence of the redox potential of Pseudomonas aeruginosa cytochrome c-551.
复制标题
铜绿假单胞菌细胞色素 c-551 氧化还原电位的 pH 依赖性。
DOI：
10.1016/0005-2728(80)90030-4
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
R. J. Williams
中科院分区：
文献类型：
--
作者：
G. Moore;G. Pettigrew;R. C. Pitt;R. J. Williams
Ps. aerosacytochromec-551随pH在5和8之间变化。pH依赖性可根据氧化形式的pKa6.2和还原形式的pKa7.3进行分析。在两种氧化态的NMR谱中也观察到相同的pKa值，并且在亚铁细胞色素的可见吸收光谱的滴定中观察到7.3的pKa。从NMR的研究，这些pKavalues已被分配到血红素丙酸groups.pH依赖的氧化还原电位的电离是可变的发生之间的细胞色素和这种变化的可能意义和基础进行了讨论。
The redox potential ofPs. aeruginosacytochromec-551 varies with pH between pH 5 and 8. The pH dependence can be analysed in terms of a pKaof 6.2 in the oxidised form and a pKaof 7.3 in the reduced form. The same pKavalues are also observed in NMR spectra of the two oxidation states and the pKaof 7.3 is observed in titration of the visible absorption spectrum of the ferrocytochrome. From the NMR studies these pKavalues have been assigned to the ionisation of one of the haem propionic acid groups.pH dependence of redox potential is of variable occurrence among cytochromes and the possible significance and basis of this variation is discussed.