Transcriptome profiling helps to elucidate the mechanisms of ripening and epidermal senescence in passion fruit (Passiflora edulia Sims).
Transcriptome profiling helps to elucidate the mechanisms of ripening and epidermal senescence in passion fruit (Passiflora edulia Sims).
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转录组分析有助于阐明百叶窗中的成熟和表皮衰老的机制（Spasiflora Edulia Sims）。
DOI：
10.1371/journal.pone.0236535
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Sun J
中科院分区：
文献类型：
--
作者：
Li C;Xin M;Li L;He X;Liu G;Li J;Sheng J;Sun J
西番莲（Passiflora edulia西姆斯）是一种重要的热带亚热带水果，属于呼吸跃变型果实，采后品质迅速劣变。为阐明紫色西番莲果实成熟和快速衰老的机制，以1-甲基环丙烯（1-MCP）和保鲜膜（PF）处理的西番莲果实为试材，对其进行了植物化学特征分析和RNA序列分析。综合功能注释和KEGG富集分析表明，淀粉和蔗糖代谢、植物激素信号转导、苯丙烷类化合物合成、类黄酮合成和类胡萝卜素合成均参与果实成熟。PF和1-MCP处理显著影响了西番莲果实采后贮藏期间的转录水平。大量差异表达的单基因（DEG）被鉴定为在采后淀粉和蔗糖代谢、植物激素信号转导和苯丙烷类化合物生物合成中显著富集。PF和1-MCP处理提高了超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）的基因表达水平和酶活性，促进了木质素的积累，降低了β-半乳糖苷酶（β-Gal）、多聚半乳糖醛酸酶（PG）和纤维素酶的活性和基因表达水平，延缓了果实衰老过程中细胞壁的降解。通过RT-qPCR验证细胞壁代谢和激素信号转导途径相关的单基因的RNA测序数据。本研究结果表明细胞壁代谢和激素信号通路与西番莲果实成熟密切相关。PF和1-MCP处理可能通过抑制乙烯信号传导，调节细胞壁代谢途径，抑制细胞壁降解。我们的研究结果表明，成熟和衰老相关的网络在西番莲果实成熟的参与，并可能建立一个基础，为今后的研究调查PF和1-MCP处理对果实成熟的影响。
Passion fruit (Passiflora edulia Sims), an important tropical and subtropical species, is classified as a respiration climacteric fruit, and its quality deteriorates rapidly after harvest. To elucidate the mechanisms involved in ripening and rapid fruit senescence, phytochemical characteristic analysis and RNA sequencing were performed in purple passion fruit with different treatments, that is, 1-methylcyclopropene (1-MCP) and preservative film (PF). Comprehensive functional annotation and KEGG enrichment analysis showed that starch and sucrose metabolism, plant hormone signal transduction, phenylpropanoid biosynthesis, flavonoid biosynthesis, and carotenoid biosynthesis were involved in fruit ripening. Treatment with PF and 1-MCP significantly affected the transcription levels of passion fruit during postharvest storage. A large number of differentially expressed unigenes (DEGs) were identified as significantly enriched in starch and sucrose metabolism, plant hormone signal transduction and phenylpropanoid biosynthesis at the postharvest stage. The PF and 1-MCP treatments increased superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) gene expression levels and enzyme activities, accelerated lignin accumulation, and decreased β-galactosidase (β-Gal), polygalacturonase (PG) and cellulose activities and gene expression levels to delay cell wall degradation during fruit senescence. The RNA sequencing data for cell wall metabolism and hormone signal transduction pathway-related unigenes were verified by RT-qPCR. The results of this study indicate that the cell wall metabolism and hormone signaling pathways are closely related to passion fruit ripening. PF and 1-MCP treatment might inhibit ethylene signaling and regulate cell wall metabolism pathways to inhibit cell wall degradation. Our results demonstrate the involvement of ripening- and senescence-related networks in passion fruit ripening and may establish a foundation for future research investigating the effects of PF and 1-MCP treatment on fruit ripening.
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影响因子：
4.3
作者：
de Oliveira, Aurelice Barbosa;de Almeida Lopes, Monica Maria;Alcantara de Miranda, Maria Raquel
通讯作者：
Alcantara de Miranda, Maria Raquel
影响因子：
4.3
作者：
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通讯作者：
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影响因子：
4.3
作者：
de Freitas-Silva, Larisse;Rodriguez-Ruiz, Marta;Corpas, Francisco J.
通讯作者：
Corpas, Francisco J.
影响因子：
6.9
作者：
Brummell, DA;Dal Cin, V;Labavitch, JM
通讯作者：
Labavitch, JM
影响因子：
6.1
作者：
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通讯作者：
Shaw, PE