Avoiding bias in fluorescence sensor readout.

Avoiding bias in fluorescence sensor readout.
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避免荧光传感器读数出现偏差。

DOI:
10.1038/s41583-023-00768-9
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发表时间:
2024
期刊:
Nature reviews. Neuroscience
影响因子:
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通讯作者:
Rusakov DA
Rusakov DA
中科院分区:
--
文献类型:
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作者:
Rusakov DA

文献摘要

相似文献

荧光指示器可以提供对大脑回路释放的信号分子的时空动态的定量洞察。然而,实验背景和实验成像设置之间的不匹配经常在这样的测量中引入意外的误差和偏差。认识到这种不匹配,应该有助于研究人员得出公正的估计。
Fluorescent indicators can provide quantitative insights into the spatiotemporal dynamics of signalling molecules released by brain circuits. However, a mismatch between the experimental context and the experimental imaging settings often introduces unexpected errors and biases in such measurements. Appreciating this mismatch should help researchers to arrive at unbiased estimates.