Cell cycle regulation in patients with intestinal metaplasia at the gastro–oesophageal junction
Cell cycle regulation in patients with intestinal metaplasia at the gastro–oesophageal junction
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胃食管交界处肠化生患者的细胞周期调节
DOI：
10.1136/mp.56.6.313
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
S. Riley
中科院分区：
文献类型：
--
作者：
N. J. Trudgill;S. Suvarna;J. Royds;S. Riley
背景/目标：食管腺癌的发病率正在迅速增加，这可能与胃食管交界处（GOJ）存在肠上皮化生（IM）有关。最近的研究已经区分了GOJ处的IM的两种亚型：短段巴雷特食管（SSBO）和正常鳞状-柱状连接处的IM（IMNSCJ）。由于细胞周期调节因子的异常表达在癌症和癌前状态中很常见，因此在GOJ IM患者中研究了细胞周期调节。研究方法：从SSBO（10例）、IMNSCJ（14例）、正常SCJ伴（14例）和不伴（12例）炎症、常规Barrett食管（BO）（12例）和食管腺癌（12例）患者中确定活检样本和切除材料。切片用p21、p27、p53、Ki 67、细胞周期蛋白D1和c-erbB 2的抗体染色，并由两名观察员使用预定标准独立评估。结果：食管腺癌组织中c-erbB 2、p53、p27、Ki 67均呈高表达。BO患者c-erbB 2阳性表达，其他标志物阴性表达。IMNSCJ患者中cyclin D1的表达显著增加。IMNSCJ患者和SSBO患者中所有其他标志物的表达相似。在SSBO和IMNSCJ患者中，Cyclin D1和c-erbB-2共表达，并且它们的表达与p53和p21的存在相关。结论：虽然SSBO（胃食管反流）和IMNSCJ（幽门螺杆菌感染）的病因不同，但细胞周期反应相似，两者都可能具有恶性潜力。
Background/Aims: The incidence of oesophageal adenocarcinoma is increasing rapidly and this may be related to the presence of intestinal metaplasia (IM) at the gastro–oesophageal junction (GOJ). Recent studies have distinguished two subtypes of IM at the GOJ: short segment Barrett’s oesophagus (SSBO) and IM at a normal squamo–columnar junction (IMNSCJ). Because abnormal expression of cell cycle regulators is common in cancer and precancerous states, cell cycle regulation was studied in patients with IM at the GOJ. Methods: Biopsy samples and resected materials were identified from patients with SSBO (10), IMNSCJ (14), a normal SCJ with (14) and without (12) inflammation, conventional Barrett’s oesophagus (BO) (12), and oesophageal adenocarcinoma (12). Sections were stained with antibodies to p21, p27, p53, Ki67, cyclin D1, and c-erbB2 and were assessed independently by two observers, using predetermined criteria. Results: Patients with oesophageal adenocarcinoma showed high expression of c-erbB2, p53, p27, and Ki67. Patients with BO showed expression of c-erbB2 but little expression of other markers. Greatly increased expression of cyclin D1 was seen in patients with IMNSCJ. The expression of all other markers was similar in patients with IMNSCJ and those with SSBO. Cyclin D1 and c-erbB-2 were coexpressed in patients with SSBO and IMNSCJ, and their expression was associated with the presence of p53 and p21. Conclusions: Although the proposed aetiologies of SSBO (gastro–oesophageal reflux) and IMNSCJ (Helicobacter pylori infection) differ, the cell cycle response is similar and both may have malignant potential.