Molecularly imprinted stir bar sorptive extraction coupled with high performance liquid chromatography for trace analysis of sulfa drugs in complex samples.
Molecularly imprinted stir bar sorptive extraction coupled with high performance liquid chromatography for trace analysis of sulfa drugs in complex samples.
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DOI：
10.1016/j.talanta.2011.03.041
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发表时间：
2011-07
期刊：
影响因子：
6.1
通讯作者：
Zhigang Xu;Chaoyong Song;Yuling Hu;Gongke Li
中科院分区：
文献类型：
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作者：
Zhigang Xu;Chaoyong Song;Yuling Hu;Gongke Li
制备了一种新型磺胺二甲嘧啶分子印迹聚合物（MIP）涂层搅拌棒，用于生物样品中8种磺胺类药物的吸附萃取。该方法的相对标准偏差（RSD）为6.7%（n= 10）。采用扫描电镜、红外光谱、热重分析和耐溶剂性研究等方法对其进行了表征。非印迹聚合物（NIP）涂层用于比较。详细评价了MIP涂层的吸附能力和选择性。MIP涂层比NIP涂层具有更高的吸附能力和选择性。在甲苯中，MIP涂层的饱和吸附量是NIP涂层的4.6倍。即使在0.2 μg/L的低浓度下，MIP涂层搅拌棒吸附萃取后也可以检测到磺胺二甲嘧啶。MIP涂层也表现出选择性吸附能力的模板类似物。建立了MIP涂层搅拌棒吸附萃取-高效液相色谱法测定生物样品中8种磺胺类药物的方法。对提取溶剂、提取时间、解吸溶剂、解吸时间和搅拌速度等提取条件进行了优化。8种磺胺类药物的线性范围分别为1.0 ~ 100 μg/L和2.0 ~ 100 μg/L。方法的检出限为0.20-0.72 μg/L。该方法用于猪肉、肝脏和鸡肉样品中磺胺类药物的多残留分析，回收率均较好。
A novel sulfamethazine molecularly imprinted polymer (MIP)-coated stir bar for sorptive extraction of eight sulfa drugs from biological samples was prepared. The MIP-coating was about 20 μm thickness with the relative standard deviation (RSD) of 6.7% (n= 10). It was characterized by scanning electron microscope, infrared spectrum, thermogravimetric analysis, and solvent-resistant investigation, respectively. The non-imprinted polymer (NIP)-coating was used for comparison. The adsorptive capacity and selectivity of MIP-coating were evaluated in detail. The MIP-coating showed higher adsorption capability and selectivity than the NIP-coating. The saturated adsorption amount of the MIP-coating was 4.6 times over that of the NIP-coating in toluene. Sulfamethazine could be detected after the MIP-coated stir bar sorptive extraction even at a low concentration of 0.2 μg/L. The MIP-coating also exhibited selective adsorption ability to analogues of the template. A method for the determination of eight sulfa drugs in biological samples by MIP coated stir bar sorptive extraction coupled with high performance liquid chromatography (HPLC) was developed. The extraction conditions, including extraction solvent, extraction time, desorption solvent, desorption time and stirring speed, were optimized. The linear ranges were 1.0–100 μg/L and 2.0–100 μg/L for eight sulfonamides, respectively. The detection limits were within the range of 0.20–0.72 μg/L. The method was successfully applied to simultaneous multi-residue analysis of eight sulfonamides in spiked pork, liver and chicken samples with the satisfactory recoveries.