Is HPV-18 present in human breast cancer cell lines?
Is HPV-18 present in human breast cancer cell lines?
复制标题
人类乳腺癌细胞系中是否存在 HPV-18？
DOI：
10.1038/sj.bjc.6605671
复制
发表时间：
2010
影响因子：
8.8
通讯作者：
Liu,X
中科院分区：
文献类型：
--
作者：
Peran,I;Riegel,A;Dai,Y;Schlegel,R;Liu,X
Lawson et al, 2009) were particularly provocative. The authors claimed that both primary human breast cancers and two well-characterised breast cancer cell lines (MDA-MB-175VII and SK-Br-3) contained HPV-18, a type of HPV found with increased frequency in adenocarcinomas (Iwasawa et al, 1996; Burk et al, 2003).To further characterise the MDA-MB-175VII and SK-Br-3 cell lines for HPV-18 gene content and expression, we performed PCR and RT–PCR to detect viral DNA and mRNA. We also included two cell lines as controls. The HeLa cervical cancer cell line contains HPV-18 and was used as a positive control and the C33 cervical cancer cell line contains no detectable HPV genomes. All cell lines were grown in 10% FBS DMEM media to approximately 85% confluency and then harvested for DNA and RNA isolation, after which we performed standard PCR and RT–PCR reactions using the indicated primer sets for the early, late, and non-coding regions of HPV-18 (see Figure 1A). For reference, we also used the exact L1
