The inhibition of synthesis of a beta-chemokine, monocyte chemotactic protein-1 (MCP-1) by progesterone
The inhibition of synthesis of a beta-chemokine, monocyte chemotactic protein-1 (MCP-1) by progesterone
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DOI：
10.1006/bbrc.1997.7502
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发表时间：
1997-10-20
影响因子：
3.1
通讯作者：
Riley, SC
中科院分区：
文献类型：
--
作者：
Kelly, RW;Carr, GG;Riley, SC
对生殖组织中趋化因子的控制还没有得到很好的描述。孕酮在许多生殖过程中起着重要的作用，孕酮和免疫系统之间的相互作用已经被假设。MCP-1是一种β趋化因子，能吸引和激活巨噬细胞，控制血管平滑肌细胞，并能调节T辅助细胞细胞因子的产生。单核细胞趋化蛋白-1也可能在排卵和分娩等生殖过程中发挥作用。单核细胞趋化蛋白-1的合成受到转录因子核因子-kappa B的刺激，并被糖皮质激素抑制，但生殖组织中孕酮控制的相关性尚不清楚。研究了孕酮对绒毛蜕膜细胞和表达雌激素不敏感孕酮受体的乳腺癌细胞株T47D分泌的影响。一种合成的孕激素(甲羟孕酮醋酸酯)抑制绒毛蜕膜细胞产生MCP-1；这种抑制作用被抗孕激素RU486逆转。IL-1可刺激T47D细胞释放MCP-1，而孕酮可抑制这种产生，其ED50小于10(-9)M。糖皮质激素(地塞米松)对T47D细胞释放MCP-1无影响，提示在此条件下糖皮质激素受体介导的反应受到损害。这些结果表明，在生殖组织中，孕酮对免疫系统的间接影响是可能的，即孕酮对上皮细胞或成纤维细胞的最初作用将传递到白细胞。(C)1997年学术出版社。
The control of chemokines in reproductive tissues has not been well characterised. Progesterone plays a major part in many reproductive processes and an interaction between progesterone and the immune system has been postulated. MCP-1 is a beta chemokine that attracts and activates macrophages, controls vascular smooth muscle cells, and can modulate T helper cell cytokine production. MCP-1 may also play a role in reproductive processes such as ovulation and parturition. MCP-1 synthesis is stimulated by the transcription factor NF-kappa B and is inhibited by glucocorticoid but the relevance of progesterone control in reproductive tissue is unknown. The effects of progesterone on the production in both choriodecidual cells and a breast cancer cell line T47D, which expresses an oestrogen insensitive progesterone receptor, were investigated. A synthetic progestin (medroxyprogesterone acetate) inhibits choriodecidual cell production of MCP-1; this inhibition was reversed by the antiprogestin RU486. MCP-1 release from T47D cells can be stimulated by IL-1 and this production is inhibited by progesterone with an ED50 of less than 10(-9) M. A glucocorticoid (dexamethasone) had no effect on MCP-1 release in this system, suggesting that glucocorticoid receptor-mediated responses were impaired under these conditions. These results demonstrate that an indirect effect of progesterone on the immune system is possible in reproductive tissues, whereby the initial effect of progesterone on epithelial or fibroblast cells would be transmitted to leukocytes. (C) 1997 Academic Press.