V lambda-light chain genes reconstitute immune responses to defined carbohydrate antigens or haptens by utilizing different VH genes.
V lambda-light chain genes reconstitute immune responses to defined carbohydrate antigens or haptens by utilizing different VH genes.
复制标题
V lambda-轻链基因利用不同的 VH 基因重建针对特定碳水化合物抗原或半抗原的免疫应答。
DOI：
10.1016/s0161-5890(96)00095-8
复制
发表时间：
1996
影响因子：
3.6
通讯作者：
Bona,CA
中科院分区：
文献类型：
--
作者：
Bot,A;Nangpal,A;Pricop,L;Bogen,B;Kaushik,A;Bona,CA
在基因操作的κ缺陷和λ2转基因小鼠中研究了λ轻链对外周B细胞库发育和产生针对半抗原和多糖抗原的特异性抗体的贡献。结果清楚地证明了在不存在k-轻链的情况下的非化学计量VH基因家族表达，并表明在外周B细胞库中表达的VH和Vλ基因之间的非随机配对。在Vlλ+抗体的情况下，VH基因利用的转变在对β2-6果聚糖和TNP半抗原的应答中是明显的。这些观察结果表明，在VKgenes的情况下，补偿机制的可用性，并与VH基因家族表达由VH基因座内的遗传因素控制的假设是一致的。此外，来自VH基因座外的遗传因素，即有限的可用轻链多样性，可能导致外周B细胞库中VH基因利用的转变。
The contribution of the λ-light chain to the development of peripheral B cell repertoire and generation of specific antibodies to haptens and polysaccharide antigens was studied in genetically manipulated kappa-deficient and λ2-transgenic mice. The results clearly demonstrate a non-stoichiometric VHgene family expression in the absence of k-light chain and suggest a non-stochastic pairing between VHand Vλgenes, expressed in the peripheral B cell repertoire. A shift in VHgene utilization in the case of Vlλ+antibodies was evident in response to β2–6 fructosan and TNP hapten. These observations demonstrate the availability of compensatory mechanisms in the absence of VKgenes and are consistent with the hypothesis that VHgene family expression is controlled by genetic factors from inside the VHlocus. Furthermore, genetic factors from outside the VHlocus, namely restricted available light chain diversity, may lead to a shift in VHgene utilization in the peripheral B cell repertoire.