A developmentally regulated Streptomyces endoribonuclease resembles ribonuclease E of Escherichia coli
A developmentally regulated Streptomyces endoribonuclease resembles ribonuclease E of Escherichia coli
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DOI：
10.1046/j.1365-2958.1997.5311904.x
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发表时间：
1997-09-01
影响因子：
3.6
通讯作者：
Cohen, SN
中科院分区：
文献类型：
--
作者：
Hagege, JM;Cohen, SN
本文报道了链霉菌S.和S.腔肠菌是一种形态复杂的革兰氏阳性土壤细菌，含有发育调节的核糖核酸内切酶活性（在此称为RNase ES），其功能和免疫学上类似于大肠杆菌RNase E。在链霉菌细胞中，RNA I -ColE 1型质粒复制的反义阻遏物-在被RNA酶E攻击的位点被切割。在S.青色素细胞提取物。在SDS/聚丙烯酰胺凝胶中以70 kDa迁移的链霉菌肽与RNase E底物结合，并与三种单独的抗RNase E单克隆抗体反应;内切核酸裂解活性与免疫反应性70 kDa肽共纯化。我们表明，RNase ES活性调节链霉菌的生命周期：活性增加，细胞从指数增长到静止期在液体培养，或从菌丝生长到孢子形成的固体培养基。而干扰S.腔棘鱼在其生命周期后期的发育并没有阻止这种与发育相关的RNase ES活性的增加，这种增加被一种突变（bldA）阻断，该突变干扰了早期的形态和生理分化。
We report that the Streptomyces species S. lividans and S. coelicolor, morphologically complex Grampositive soil bacteria, contain a developmentally regulated endoribonuclease activity (here named RNase ES) that functionally and immunologically resembles Escherichia coli RNase E. In Streptomyces cells, RNA I - the antisense repressor of replication of ColE1-type plasmids - is cleaved at sites attacked by RNase E. A Mg2+-dependent endonuclease that produces RNase E-like cleavages in RNA I and 9S ribosomal RNA was identified in S. lividans cell extracts. A Streptomyces peptide migrating at 70kDa in SDS/polyacrylamide gels binds to RNase E substrates and reacts with three separate anti-RNase E monoclonal antibodies; the endonucleolytic cleavage activity co-purified with the immunoreactive 70 kDa peptide. We show that RNase ES activity is regulated during the Streptomyces life cycle: activity increased as cells progressed from exponential growth to stationary phase in liquid culture, or from mycelial growth to sporulation on solid media. While mutations that interfere with S. coelicolor development late in its life cycle did not prevent this developmentally associated increase in RNase ES activity, the increase was blocked by a mutation (bldA) that interferes early with both morphological and physiological differentiation.