The expression and regulation of follistatin and a follistatin-like gene during avian somite compartmentalization and myogenesis
The expression and regulation of follistatin and a follistatin-like gene during avian somite compartmentalization and myogenesis
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DOI：
10.1006/dbio.1996.0223
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发表时间：
1996-09-15
影响因子：
2.7
通讯作者：
Christ, B
中科院分区：
文献类型：
--
作者：
Amthor, H;Connolly, D;Christ, B
我们报道了两个结构上相关的基因Follistatin和Follistatin-like(Flik)在禽类胚胎体节中的正常和实验改变的表达。在正常鸡胚中，卵泡抑素首先在背外侧体节的细胞中表达。在体节成熟过程中，背内侧的细胞也表达该基因。在皮肌节内，似乎只有肌肉前体是卵泡抑素阳性的。肢体和舌肌的迁移前体以及肌节细胞均有Follistatin的表达。操纵实验表明，脊索信号可以抑制卵泡抑素在体节中的表达。这种效应可以通过声波刺猬蛋白来模仿。Flik在体节的背内侧室中表达，随后在肌节中表达。与卵泡抑素不同，Flik的表达需要神经管发出的信号。脊索影响不会改变Flik的表达。这两个基因的表达不依赖于中间或外侧中胚层的信号。由于这两个基因的产物都被认为是在原肠形成和神经化过程中拮抗转化生长因子-β超家族蛋白，我们推测在肌肉发生过程中，卵泡抑素和flk抵消了相关分子对肌肉分化的抑制作用。(C)1996年学术出版社。
We report on the normal and experimentally altered expression of two structurally related genes, Follistatin and Follistatin-like (Flik), in the somites of avian embryos. In normal chick embryos, Follistatin expression can first be seen in the cells of the dorsolateral somite quarter. During somite maturation, the cells of the dorsomedial quarter also express this gene. Within the dermomyotome it seems that only the muscle precursors are Follistatin-positive. The migrating precursors of limb and tongue muscle as well as the myotome cells show Follistatin expression. The manipulation experiments reveal that the expression of Follistatin in the somites can be inhibited by notochord signals. This effect can be mimicked by sonic hedgehog protein. Flik is expressed in the dorsomedial compartment of the somite and later on in the myotome. Unlike follistatin, Flik expression requires signals emanating from the neural tube. Notochordal influences do not alter Flik expression. The expression of both genes does not depend on signals of intermediate or lateral mesoderm. Since the products of both genes are proposed to antagonize TGF-beta superfamily proteins during gastrulation and neuralization, we postulate that during myogenesis follistatin and flik counteract inhibiting effects of related molecules on muscle differentiation. (C) 1996 Academic Press, Inc.