Remodeling of yeast genome expression in response to environmental changes
Remodeling of yeast genome expression in response to environmental changes
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DOI：
10.1091/mbc.12.2.323
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发表时间：
2001-02-01
影响因子：
3.3
通讯作者：
Young, RA
中科院分区：
文献类型：
--
作者：
Causton, HC;Ren, B;Young, RA
我们使用全基因组表达分析来探索酿酒酵母中的基因表达如何响应细胞外环境的各种变化而重塑，包括温度，氧化，营养，pH和渗透压的变化。结果表明，超过一半的基因组参与了对环境变化的各种反应，并确定了由每种条件诱导和抑制的基因的全局集合。这些数据表明，在这些反应中存在大量以前未表征的基因，并揭示了环境反应中常见的一个特征，涉及类似于10%的酵母基因。MSN 2/MSN 4突变体的表达分析结果支持Msn 2/Msn 4激活剂诱导对环境变化的共同反应的模型。这些结果提供了一个全球性的描述的转录响应环境变化，并扩展我们的理解激活剂在影响这种反应的作用。
We used genome-wide expression analysis to explore how gene expression in Saccharomyces cerevisiae is remodeled in response to various changes in extracellular environment, including changes in temperature, oxidation, nutrients, pH, and osmolarity. The results demonstrate that more than half of the genome is involved in various responses to environmental change and identify the global set of genes induced and repressed by each condition. These data implicate a substantial number of previously uncharacterized genes in these responses and reveal a signature common to environmental responses that involves similar to 10% of yeast genes. The results of expression analysis with MSN2/MSN4 mutants support the model that the Msn2/Msn4 activators induce the common response to environmental change. These results provide a global description of the transcriptional response to environmental change and extend our understanding of the role of activators in effecting this response.