Identification of HEV in symptom-free migrants and environmental samples in Italy
Identification of HEV in symptom-free migrants and environmental samples in Italy
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DOI：
10.1111/jvh.12038
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发表时间：
2013-06-01
影响因子：
2.5
通讯作者：
De Donno, A.
中科院分区：
文献类型：
--
作者：
Idolo, A.;Serio, F.;De Donno, A.
戊型肝炎病毒（HEV）被认为是一种新兴的病原体在工业化国家。对抵达意大利南部海岸的无症状移民粪便样本和环境样本中HEV基因型的发生进行了调查。序列分析用于比较人类和环境基因型。共分析了40份粪便标本、12份未经处理的城市污水样品、12份经处理的城市污水样品和12份地表水样品。采用切向流超滤技术从水样中浓缩病毒。巢式RT-PCR检测HEV RNA。对病毒分离株进行测序和遗传学表征。两个（5%）的40个粪便样本测试被发现是阳性的HEV RNA（G1和G3基因型）。在未处理的污水和地表水样品中分别有25%（3/12）和25%（3/12）检出病毒，所有分离株均为G3基因型。经处理的污水样品均未发现HEV RNA阳性。在两名无症状受试者的粪便中检测到该病毒，表明无病毒的HEV携带者作为人类储存库的潜在作用。在未经处理的污水中检测到G3 HEV菌株，与在其他欧洲国家进行的类似研究中观察到的结果相同，但与最近在意大利进行的另一项研究不同。此外，我们的研究结果显示了第一例从新鲜的地表沃茨中分离出的HEV。
Hepatitis E virus (HEV) is considered an emerging pathogen in industrialized countries. The occurrence of HEV genotypes in samples of faeces from asymptomatic migrants arriving on the coasts of South Italy and environmental samples was investigated. Analyses of sequences were used to compare human and environmental genotypes. A total of 40 stool specimens, 12 samples of untreated urban sewage, 12 samples of treated urban sewage and 12 samples of surface water were analysed. Viruses were concentrated from water samples by the tangential flow ultrafiltration technique. The presence of HEV RNA was detected by nested RT-PCR. Viral isolates were sequenced and phylogenetically characterized. Two (5%) of the 40 faecal samples tested were found to be positive for HEV RNA (G1 and G3 genotypes). The virus was detected in 25% (3/12) of the untreated sewage samples and 25% (3/12) of the surface water samples: all isolates belonged to G3 genotype. None of the treated sewage samples were found to be HEV RNA positive. The virus was detected in the faeces of two asymptomatic subjects, suggesting a potential role for symptom-free HEV carriers as a human reservoir. G3 HEV strains were detected in the untreated sewage, as observed in similar studies conducted in other European countries but differing from another study conducted in Italy recently. Moreover, our results show the first case of HEV isolated from fresh surface waters.