Correlation between cellular ATP level and bile excretion in the rat liver.
Correlation between cellular ATP level and bile excretion in the rat liver.
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细胞 ATP 水平与大鼠肝脏胆汁排泄之间的相关性。
DOI：
10.1097/00007890-198501000-00005
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发表时间：
1985
期刊：
影响因子：
6.2
通讯作者：
K. Tagawa
中科院分区：
文献类型：
--
作者：
W. Kamiike;M. Nakahara;K. Nakao;M. Koseki;T. Nishida;Y. Kawashima;F. Watanabe;K. Tagawa
本文研究了大鼠肝脏细胞水平的三磷酸腺苷(ATP)对胆汁排泄的影响。缺血时，细胞内三磷酸腺苷水平迅速下降，胆汁流量在5分钟内停止。L-乙硫氨酸腹腔注射给药降低胆汁流率，降低细胞内三磷酸腺苷水平。在肝脏灌流系统中，胆汁流量与细胞内ATP水平之间的相关性得到了证实。缺氧灌流时，三磷酸腺苷水平和胆汁流量的变化与缺血时相同。两者复氧恢复率均随缺氧灌注期的延长而降低。在有氧条件下的灌流过程中，给予不同浓度的呼吸抑制剂氰化钾，使细胞内的三磷酸腺苷不同程度地降低，从而相应地抑制胆汁的排泄。胆汁流率的动力学分析显示细胞内三磷酸腺苷水平呈米氏曲线。L-乙硫氨酸处理大鼠肝脏和氰化钾灌流大鼠肝脏胆汁流量的表观Km值分别为1.0和1.6 mM，Vmax分别为4.1和2.5微升/分/克肝脏。胆汁主要成分磷脂、胆固醇、牛磺胆酸浓度随ATP水平的降低而升高，但总产量随ATP水平的降低而下降，随着ATP水平的恢复而恢复到正常范围。因此，实验表明，通过监测反映细胞水平的三磷酸腺苷的胆汁流量，可以简单地评估肝脏损伤的程度。
The influence of the cellular level of adenosine triphosphate (ATP) in the liver on bile excretion was studied in rats. In ischemia, the cellular ATP level decreased rapidly--and, concomitantly, bile flow stopped within 5 min. Administration of L-ethionine i.p. to rats reduced the bile flow rate with decrease in the cellular ATP level. The correlation between the bile flow rate and the cellular ATP level was confirmed in a liver perfusion system. On anoxic perfusion, the ATP level and bile flow rate changed in the same manner as in ischemia. The recovery rates of both on reoxygenation decreased with increase in the anoxic perfusion period. During perfusion under oxygenated conditions, decrease in cellular ATP to various levels by infusion of various concentrations of potassium cyanide, an inhibitor of respiration, resulted in corresponding and concomitant suppression of bile excretion. Kinetic analysis of the bile flow rate revealed a Michaelis-Menten-type curve for the cellular ATP level. The apparent Kms for ATP of bile flow rate in L-ethionine-treated rat liver and liver perfused with potassium cyanide were 1.0 and 1.6 mM, and their Vmax values were 4.1 and 2.5 microliter/min/g liver, respectively. The concentrations of main bile components, such as phospholipids, cholesterol, and taurocholate increased, but their total outputs decreased with decrease in the ATP level, and returned to the normal range with recovery of the ATP level. Thus, it was shown experimentally that the extent of hepatic injury can be assessed simply by monitoring the bile flow rate, which reflects the cellular level of ATP.