Fluorescence Polarization: Recent Bioanalytical Applications, Pitfalls, and Future Trends
Fluorescence Polarization: Recent Bioanalytical Applications, Pitfalls, and Future Trends
复制标题
DOI：
10.1007/4243_2008_021
复制
发表时间：
2008-01-01
期刊：
影响因子：
--
通讯作者：
Le, X. Chris
中科院分区：
文献类型：
--
作者：
Goulko, Alevtina A.;Zhao, Qiang;Le, X. Chris
荧光偏振 (FP) 对分子大小的变化敏感，因此利用分子相互作用的测定特别有用。 FP 免疫测定依赖于抗体-抗原相互作用，广泛用于临床分析、食品分析和环境监测。其他 FP 技术的发展，如受体-配体和肽-配体亲和力测定、适体亲和力测定、磷酸化学物质的固定金属测定、单核苷酸多态性测定和毛细管电泳激光诱导 FP 测定，进一步将 FP 技术的应用扩展到药物发现、蛋白质-DNA 相互作用、疾病诊断和生化研究。配备偏振光学器件的酶标仪的使用导致采用 FP 作为高通量筛选测定的读出模式。本章简要介绍了 FP 的基本原理，并总结了 FP 的一些近期生物分析应用。它还讨论了 FP 检测的主要优点、局限性和未来前景。
Fluorescence polarization (FP) is sensitive to changes in molecular size and, therefore, assays that make use of molecular interactions are particularly useful. FP immunoassays, which rely on antibody-antigen interactions are widely used for clinical analysis, food analysis, and environmental monitoring. Development of other FP techniques, such as receptor-ligand and peptide-ligand affinity assays, aptamer affinity assays, immobilized metal assays for phospho chemicals, assays for single nucleotide polymorphisms, and capillary electrophoresis laser-induced FP assays, have further extended the applications of FP techniques to drug discovery, protein-DNA interactions, disease diagnostics, and biochemical research. The use of microplate readers equipped with polarizing optics has led to the adoption of FP as a readout mode for high-throughput screening assays. This chapter briefly describes the basic principle of FP and summarizes some of the recent bioanalytical applications of FP. It also discusses the main advantages, limitations, and future prospects of FP assays.