Improving power production in acetate-fed microbial fuel cells via enrichment of exoelectrogenic organisms in flow-through systems
Improving power production in acetate-fed microbial fuel cells via enrichment of exoelectrogenic organisms in flow-through systems
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DOI：
10.1016/j.bej.2009.08.008
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发表时间：
2009-12-15
影响因子：
3.9
通讯作者：
Andras, Calin
中科院分区：
文献类型：
--
作者：
Borole, Abhijeet P.;Hamilton, Choo Y.;Andras, Calin
一个产电，生物膜形成的微生物群落富集在一个乙酸盐进料的微生物燃料电池（MFC）使用的流通阳极耦合到一个空气阴极。使用具有低电极间距、具有最小死体积的高比电极表面积和控制外部电阻的MFC设计。此外，采用碳源的连续进料，并且MFC在间歇性高流量下操作，以能够在6个月的时间内去除非生物膜形成生物体。当与铁氰化物阴极耦合时，使用修改的设计和操作条件富集的聚生体导致净阳极体积的功率密度为345 W m（-3）（3650 mW m（-2））。富集的聚生体包括β、del、γ-变形菌门、拟杆菌门和厚壁菌门。红环菌科和伯克霍尔德菌科的成员（固氮螺菌属（49%），食酸菌属（11%）和丛毛单胞菌属（7%）），占主导地位的微生物财团。变性梯度凝胶电泳（DGGE）分析的基础上选择性引物的古生菌表明存在非常少的产甲烷菌。通过连续进料限制碳源的输送，对应于流通式空气阴极MFC中允许的最大阴极氧化速率，导致库仑效率达到88 +/-5.7%。(C)2009爱思唯尔B V保留所有权利。
An exoelectrogenic, biofilm-forming microbial consortium was enriched in an acetate-fed microbial fuel cell (MFC) using a flow-through anode coupled to an air-cathode. An MFC design with low electrode spacing, high specific electrode surface area with minimal dead volume and control of external resistance was used. In addition, continuous feeding of carbon source was employed and the MFC was operated at intermittent high flows to enable removal of non-biofilm-forming organisms over a period of 6 months. The consortium enriched using the modified design and operating conditions resulted in a power density of 345 W m(-3) of net anode volume (3650 mW m(-2)), when coupled to a ferricyanide cathode. The enriched consortium included beta, del, gamma-Proteobacteria, Bacteroidetes and Firmicutes. Members of the order Rhodocyclaceae and Burkholderiaceae (Azospira sp. (49%), Acidovorax sp. (11%) and Comamonas sp. (7%)), dominated the microbial consortium. Denaturing gradient gel electrophoresis (DGGE) analysis based on primers selective for archaea indicated presence of very few methanogens. Limiting the delivery of the carbon source via continuous feeding corresponding to the maximum cathodic oxidation rates permitted in the flow-through, air-cathode MFC resulted in coulombic efficiencies reaching 88 +/- 5.7%. (C) 2009 Elsevier B V All rights reserved.