Heritability of shell pigmentation in the Pacific oyster, Crassostrea gigas
Heritability of shell pigmentation in the Pacific oyster, Crassostrea gigas
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DOI：
10.1016/j.aquaculture.2008.09.022
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发表时间：
2009-01-17
期刊：
影响因子：
4.5
通讯作者：
Langdon, Christopher J.
中科院分区：
文献类型：
--
作者：
Evans, Sanford;Camara, Mark D.;Langdon, Christopher J.
太平洋牡蛎（Crassostrea gigas）是一种具有相当重要经济意义的物种，是全球养殖水生动物物种产量最高的物种之一。为了提高太平洋牡蛎在半壳市场上作为“单壳”出售的价值，我们通过估计太平洋牡蛎总左壳色素的广义和狭义遗传力，探讨了通过选择性育种来改变壳色素的可行性。千兆。26个全同胞家庭来自父母收集的归化人口在Dabob湾，华盛顿州，产卵在孵化场，并提出了在潮间带环境2年。在收获时，我们从每个家庭的贝壳中取样，并使用数字图像分析来量化它们的总左壳色素沉着。我们估计基于全同胞组内相关的广义遗传力和基于双亲-后代回归的狭义遗传力分别为0.91 +/- 038和0.59 +/- 0.19。我们进一步研究了全同胞家庭中个体之间的色素沉着水平的分布，发现在大多数家庭中，总壳色素沉着的家庭内变异很高，并且是正常和连续分布的。然而，两个家系的后代以3：1的比例分离为表型上不同的“较亮”和“较暗”壳组（chi（2），P>0.766），这支持了一个假设，即在这些家系中，一个单一的主基因分离，“较亮”等位基因对“较暗”等位基因是显性的。我们的结论是，选择育种作用于这种高加性遗传方差应有效地改变总壳色素，并需要进一步的工作，以确认存在一个假定的主基因影响总壳色素在C。千兆。(C)2008 Elsevier B. V.保留所有权利。
The Pacific oyster (Crassostrea gigas) is a species of considerable economic importance, with among the highest global production of any cultured aquatic animal species. In the interest of increasing the value of Pacific oysters sold as "singles" for the half-shell market, we explored the feasibility of modifying shell pigmentation through selective breeding by estimating both the broad- and narrow-sense heritability of total left-shell pigmentation in C. gigas. Twenty-six full-sib families derived from parents collected from a naturalized population in Dabob Bay, WA, were spawned in the hatchery and raised in an intertidal environment for 2 years. At harvest, we sampled shells from each family and quantified their total left-shell pigmentation using digital image analysis. We estimated broad-sense heritability based on full-sib intraclass correlations and narrow-sense heritability based on midparent-offspring regression as 0.91 +/- 038 and 0.59 +/- 0.19, respectively. We further examined the distributions of pigmentation levels among individuals within full-sib families and found high within-family variation in total shell pigmentation that in the majority of families was normally and continuously distributed. However, offspring within two families segregated into phenotypically distinct "lighter" and "darker" shell groups in a 3:1 ratio (chi(2), P>0.766) supporting the hypothesis that a single major gene is segregating in these families with the "light" allele being dominant over the "dark" allele. We conclude that selective breeding acting on this high additive genetic variance should be effective in altering total shell pigmentation and that further work is needed to confirm the existence and mode of inheritance of a putative major gene affecting total shell pigmentation in C. gigas. (C) 2008 Elsevier B.V. All rights reserved.