The cotranslational integration of membrane proteins into the phospholipid bilayer is a multistep process
The cotranslational integration of membrane proteins into the phospholipid bilayer is a multistep process
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DOI：
10.1016/s0092-8674(00)81115-0
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发表时间：
1996-05-03
期刊：
影响因子：
64.5
通讯作者：
Johnson, AE
中科院分区：
文献类型：
--
作者：
Do, H;Falcone, D;Johnson, AE
在新生蛋白与内质网膜共译整合过程中，跨膜（TM）序列从Sec61 α、TRAM和其他蛋白质形成的水孔中移动到非极性脂质双分子层中。光交联表明，这种运动涉及到TM结构域通过三种不同的蛋白质环境的顺序传递：一个毗邻Sec61 α和TRAM，两个毗邻TRAM，这对TM结构域的运动施加了不同的限制。此外，在翻译结束之前，TM序列不允许从最后的tran -近端部位扩散到双分子层。因此，共译整合与翻译有关，并通过内质网的有序多步骤途径发生，内质网在结构和功能上都是多层的。
During the cotranslational integration of a nascent protein into the endoplasmic reticulum membrane, the transmembrane (TM) sequence moves out of an aqueous pore formed by Sec61 alpha, TRAM, and other proteins and into the nonpolar lipid bilayer. Photocross-linking reveals that this movement involves the sequential passage of the TM domain through three different proteinaceous environments: one adjacent to Sec61 alpha and TRAM and two adjacent to TRAM that place different restrictions on TM domain movement. In addition, the TM sequence is not allowed to diffuse into the bilayer from the final TRAM-proximal site until translation terminates. Cotranslational integration is therefore linked to translation and occurs via an ordered multistep pathway at an endoplasmic reticulum site that is multilayered both structurally and functionally.