One-pot preparation of mono-dispersed and physiologically stabilized gold colloid
One-pot preparation of mono-dispersed and physiologically stabilized gold colloid
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DOI：
10.1007/s00396-005-1339-9
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发表时间：
2005-10-01
影响因子：
2.4
通讯作者：
Nagasaki, Y
中科院分区：
文献类型：
--
作者：
Sakura, T;Takahashi, T;Nagasaki, Y
We have developed a rapid and simple method for the preparation of nearly mono-dispersed gold colloids with a fairly high concentration above 10 mM using hydroxylamine as the reducing agents, in the presence of alpha-methoxy-omega-mercaptoethyl-poly(ethylene glycol)(MeO-PEG-SH). It was found that a hydroxylamine acted not only as a reducing agent, but also as a nucleation agent under alkaline reaction conditions. Though the colloid concentration was fairly high, the dispersion stability was remarkably improved even in a high ionic strength in the range greater than 1 M NaCl, in which conventional citrate gold colloids immediately flocculate and precipitate. The obtained colloid was successfully re-dispersed in aqueous media after lyophilization. In addition, the prepared gold colloid reduced a protein adsorption significantly on its surface. Concerning these results, the obtained colloidal dispersion may be suitable for biological applications, since a regionally concentrated colloidal dispersion with dispersion stability is required for bio-labeling and bio-imaging systems.
