Systematic analysis of SNARE molecules in Arabidopsis:: Dissection of the post-Golgi network in plant cells
Systematic analysis of SNARE molecules in Arabidopsis:: Dissection of the post-Golgi network in plant cells
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DOI：
10.1247/csf.29.49
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发表时间：
2004-04-01
影响因子：
1.5
通讯作者：
Sato, MH
中科院分区：
文献类型：
--
作者：
Uemura, T;Ueda, T;Sato, MH
在所有真核细胞中，囊泡运输过程中的特异性囊泡融合是由称为SNARE（可溶性N-乙基-马来酰亚胺敏感因子附着蛋白受体）的膜相关蛋白介导的。序列分析共鉴定出54个SNARE基因（18个Qa-SNARE/Syntaxins、11个Qb-SNARE、8个Qc-SNARE、14个R-SNARE/VAMP和3个SNAP-25）。几乎所有这些基因在所有检测的组织中都普遍表达。使用绿色荧光蛋白（GFP）融合蛋白的一系列瞬时表达测定揭示了大多数SNARE蛋白位于特定的细胞内区室上：内质网6条，高尔基体9条，高尔基体网络4条，核内体2条，质膜17条，液泡前室和液泡7条，TGN/PVC/液泡2条，部分SNARE蛋白在两个或两个以上不同的细胞器中具有多重定位模式，表明这些SNARE蛋白在细胞器之间穿梭。此外，SYP 41/SYP 61-驻留区室，这被定义为TGN，并不总是位于沿着与高尔基体，这表明该区室是一个独立的细胞器不同于高尔基体。我们提出了所有亚细胞区室的SNARE蛋白的可能组合，并建议在高等植物细胞中的后高尔基体膜交通的复杂性。
In all eucaryotic cells, specific vesicle fusion during vesicular transport is mediated by membrane-associated proteins called SNAREs (solubleN-ethyl-maleimide sensitive factor attachment protein receptors). Sequence analysis identified a total of 54 SNARE genes (18 Qa-SNAREs/Syntaxins, 11 Qb-SNAREs, 8 Qc-SNAREs, 14 R-SNAREs/VAMPs and 3 SNAP-25) in the Arabidopsis genome. Almost all of them were ubiquitously expressed through out all tissues examined. A series of transient expression assays using green fluorescent protein (GFP) fused proteins revealed that most of the SNARE proteins were located on specific intracellular compartments: 6 in the endoplasmic reticulum, 9 in the Golgi apparatus, 4 in the trans-Golgi network (TGN), 2 in endosomes, 17 on the plasma membrane, 7 in both the prevacuolar compartment (PVC) and vacuoles, 2 in TGN/PVC/vacuoles, and 1 in TGN/PVC/plasma membrane.Some SNARE proteins showed multiple localization patterns in two or more different organelles, suggesting that these SNAREs shuttle between the organelles. Furthermore, the SYP41/SYP61-residing compartment, which was defined as the TGN, was not always located along with the Golgi apparatus, suggesting that this compartment is an independent organelle distinct from the Golgi apparatus. We propose possible combinations of SNARE proteins on all subcellular compartments, and suggest the complexity of the post-Golgi membrane traffic in higher plant cells.