Gene expression signature of human HepG2 cell line
Gene expression signature of human HepG2 cell line
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DOI：
10.1016/j.gene.2012.12.106
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发表时间：
2013-04-15
期刊：
影响因子：
3.5
通讯作者：
Colonna, G.
中科院分区：
文献类型：
--
作者：
Costantini, S.;Di Bernardo, G.;Colonna, G.
肝细胞癌（HCC）是世界范围内第五大常见癌症，与多种临床病理特征如基因突变和病毒感染相关。因此，许多实验室都在寻找新的推定标志物来改善HCC诊断/预后。许多分子谱研究调查了与HCC相关的基因表达变化。HepG 2代表了人肝癌的纯细胞系，由于不存在病毒感染而经常用作HCC模型。在这项研究中，我们比较基因表达谱与HepG 2（肝癌模型）和正常肝细胞芯片技术。基因的层次聚类分析表明，2646个基因显着下调HepG 2细胞相比，肝细胞，而另外3586个基因显着上调。通过使用Inflamity Pathway Analysis（IPA）程序，我们对不同表达的基因进行了分类，并研究了完整人类相互作用组中与这些基因相关的功能网络。此外，为了证实差异表达的基因以及我们的芯片数据的可靠性，我们进行了定量真实的时间RT-PCR分析的9个上调和11个下调基因，分别。总之，这项工作i）提供了人类肝癌细胞的基因特征，显示在没有任何基因突变或病毒感染的情况下，由于肝癌而改变其表达的基因，ii）与以前发表的研究相比，在我们的签名中发现的新的不同表达的基因的证据，以及iii）建议一些基因，未来的研究将关注这些基因，以了解它们是否可以用于改善HCC预后。诊断. (C)2013爱思唯尔有限公司版权所有。
Hepatocellular carcinoma (HCC) is the fifth most common cancer worldwide and is associated with various clinico-pathological characteristics such as genetic mutations and viral infections. Therefore, numerous laboratories look out for identifying always new putative markers for the improvement of HCC diagnosis/prognosis. Many molecular profiling studies investigated gene expression changes related to HCC. HepG2 represents a pure cell line of human liver carcinoma, often used as HCC model due to the absence of viral infection. In this study we compare gene expression profiles associated with HepG2 (as HCC model) and normal hepatocyte cells by microarray technology. Hierarchical cluster analysis of genes evidenced that 2646 genes significantly down-regulated in HepG2 cells compared to hepatocytes whereas a further 3586 genes significantly up-regulated. By using the Ingenuity Pathway Analysis (IPA) program, we have classified the genes that were differently expressed and studied the functional networks correlating these genes in the complete human interactome. Moreover, to confirm the differentially expressed genes as well as the reliability of our microarray data, we performed a quantitative Real time RT-PCR analysis on 9 up-regulated and 11 down-regulated genes, respectively. In conclusion this work i) provides a gene signature of human hepatoma cells showing genes that change their expression as a consequence of liver cancer in the absence of any genetic mutations or viral infection, ii) evidences new differently expressed genes found in our signature compared to previous published studies and iii) suggests some genes on which to focus future studies to understand if they can be used to improve the HCC prognosis/diagnosis. (C) 2013 Elsevier B.V. All rights reserved.