Generation of expressed sequence tags from a cDNA library of Coleus forskohlii for identification of genes involved in terpene biosynthesis
Generation of expressed sequence tags from a cDNA library of Coleus forskohlii for identification of genes involved in terpene biosynthesis
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DOI：
10.1007/s10535-015-0526-x
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发表时间：
2015-06
影响因子：
1.5
通讯作者：
Y. Fang;J. Huang;Xiao Huang;S. Chen;P. Zou;W. S. Li;Kun Yu;Yanwen Liu
中科院分区：
文献类型：
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作者：
Y. Fang;J. Huang;Xiao Huang;S. Chen;P. Zou;W. S. Li;Kun Yu;Yanwen Liu
冬青(Coleus Forskohlii)是一种用途广泛的药用植物，其主要生物活性成分为二萜类化合物Forsklin和异Forsklin。本研究旨在构建一个鉴定与萜类生物合成相关的表达序列标签的cDNA文库。福斯科利。我们构建了一个高质量的标准化全长cDNA文库，该文库达到了分离全长基因的丰度、完整性和文库含量的要求。共测定了4 224个克隆的序列，组装了2394个非基因片段，平均Unigene大小为753bp。使用基因本体论对2100个(87.7%)非基因进行了功能分类，其中1716个(71.7%)非基因被分配用于在KEGG图谱中建立路径关联。值得注意的是，单胞菌可能参与了次生代谢物的生物合成，其中鉴定出17个单胞菌，它们可能参与了萜类主干和单萜、二萜和三萜的生物合成。
Coleus forskohlii(syn.Plectranthus barbatus) is a widely used medicinal plant and its main bioactive constituents are diterpenes forskolin and isoforskolin. The present study aimed to construct a cDNA library to identify expressed sequence tags related to terpene biosynthesis inC. forskohlii. We constructed a high quality normalized full-length cDNA library which reached the requirements (abundance, integrity, and library content) for isolating full-length genes. A total of 4 224 cDNA clones were sequenced and 2 394 unigenes were assembled with an average unigene size of 753 bp. A total of 2 100 (87.7 %) unigenes were functionally classified using gene ontologies, and 1 716 (71.7 %) unigenes were assigned to establish pathway associations inKEGGmapings. Notably, 64 unigenes putatively participated in the biosynthesis of secondary metabolites, in which 17 unigenes were identified that might be involved in the biosynthesis of the terpenoid backbone and monoterpenes, diterpenes, and triterpenes.