More accurate matrix-matched quantification using standard superposition method for herbal medicines
More accurate matrix-matched quantification using standard superposition method for herbal medicines
复制标题
使用标准叠加法对草药进行更准确的基质匹配定量。
DOI：
10.1016/j.chroma.2012.07.020
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发表时间：
2012-09-07
影响因子：
4.1
通讯作者：
Li, Ping
中科院分区：
文献类型：
--
作者：
Liu, Ying;Shi, Xiao-Wei;Li, Ping
已经开发了各种分析技术用于定量测定草药（HM）中的标记化合物。一个重要的问题是基质效应，必须在不同检测的方法验证中解决。与生物液体不同，用于校准的空白基质样品通常不可用于HM。在这项工作中，提出了实用的方法，以尽量减少基质效应在HM分析。采用高效液相色谱法对三七中5种皂苷类成分进行定量分析，考察了基质效应对分析结果的影响。基质成分被发现干扰电离的目标分析物时，质谱（MS）检测。为了补偿矩阵信号抑制/增强，两个矩阵匹配的方法，标准加入法与目标敲除提取物和标准叠加法与HM提取物的开发和测试在这项工作中。结果表明，标准叠加法克服了基体效应对HMs定量分析的影响，方法简单实用。而且。当蒸发光散射检测（ELSD）用于HMs的定量分析时，观察到干扰组分干扰目标分析物的光散射，但当采用紫外检测（UV）时未显示出干扰组分。因此，HPLC-ELSD和HPLC-MS定量方法用于HMs质量控制时，应解决干扰效应问题并将其降至最低。(c)2012爱思唯尔有限公司版权所有。
Various analytical technologies have been developed for quantitative determination of marker compounds in herbal medicines (HMs). One important issue is matrix effects that must be addressed in method validation for different detections. Unlike biological fluids, blank matrix samples for calibration are usually unavailable for HMs. In this work, practical approaches for minimizing matrix effects in HMs analysis were proposed. The matrix effects in quantitative analysis of five saponins from Panax notoginseng were assessed using high-performance liquid chromatography (HPLC). Matrix components were found to interfere with the ionization of target analytes when mass spectrometry (MS) detection were employed. To compensate the matrix signal suppression/enhancement, two matrix-matched methods, standard addition method with the target-knockout extract and standard superposition method with a HM extract were developed and tested in this work. The results showed that the standard superposition method is simple and practical for overcoming matrix effects for quantitative analysis of HMs. Moreover. the interference components were observed to interfere with light scattering of target analytes when evaporative light scattering detection (ELSD) was utilized for quantitative analysis of HMs but was not indicated when Ultraviolet detection (UV) were employed. Thus, the issue of interference effects should be addressed and minimized for quantitative HPLC-ELSD and HPLC-MS methodologies for quality control of HMs. (c) 2012 Elsevier B.V. All rights reserved.