Protein Patterning by UV-Induced Photodegradation of Poly(oligo(ethylene glycol) methacrylate) Brushes
Protein Patterning by UV-Induced Photodegradation of Poly(oligo(ethylene glycol) methacrylate) Brushes
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DOI：
10.1021/la100438d
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发表时间：
2010-06-15
期刊：
影响因子：
3.9
通讯作者：
Leggett, Graham J.
中科院分区：
文献类型：
--
作者：
Ahmad, Shahrul Alang;Hucknall, Angus;Leggett, Graham J.
使用 X 射线光电子能谱 (XPS) 和原子力显微镜 (A FM) 研究了通过表面引发原子自由基转移聚合在二氧化硅上生长的抗蛋白质聚（低聚（乙二醇）甲基丙烯酸酯）（POEGMA）瓶刷膜的紫外光降解。暴露在波长 244 nm 的光下会导致刷结构中聚醚单元的损失，并产生可以用胺衍生的醛基。与天然刷相比，光降解聚合物刷的摩擦系数有所增加，这归因于异质表面膜的产生，导致通过膜变形增加能量耗散以及在表面产生新的极性官能团。通过光掩模对胶片进行曝光，产生清晰、清晰的图案。形貌图像分析表明，在曝光过程中发生了材料的物理去除，速率为 1.35 nm J(-1) cm(2)。使用荧光显微镜，研究了标记蛋白质在暴露表面上的吸附。研究发现，蛋白质强烈吸附在暴露区域，而遮蔽区域保留了其蛋白质抗性。将薄膜暴露在扫描近场光学显微镜的紫外光下，产生亚微米级的图案。这些数据表明，聚（OEGMA）刷发生了简单、快速、一步的光转换，将其从一种高度抗蛋白质的材料转变为一种吸附蛋白质并可以共价结合含胺分子的材料，并且这种光转换可以以高空间分辨率进行空间寻址。
The UV photodegradation of protein-resistant poly(oligo(ethylene glycol) methacrylate) (POEGMA) bottle-brush films, grown on silicon oxide by surface-initiated atom radical transfer polymerization, was studied using X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (A FM). Exposure to light with a wavelength of 244 nm caused a loss of polyether units from the brush structure and the creation of aldehyde groups that could be derivatized with amines. An increase was measured in the coefficient of friction of the photodegraded polymer brush compared to the native brush, attributed to the creation of a heterogeneous surface film, leading to increased energy dissipation through film deformation and the creation of new polar functional groups at the surface. Exposure of the films through a photomask yielded sharp, well-defined patterns. Analysis of topographical images showed that physical removal of material occurred during exposure, at a rate of 1.35 nm J(-1) cm(2). Using fluorescence microscopy, the adsorption of labeled proteins onto the exposed surfaces was studied. It was found that protein strongly adsorbed to exposed areas, while the masked regions retained their protein resistance. Exposure of the film to UV light from a scanning near-field optical microscope yielded submicrometer-scale patterns. These data indicate that a simple, rapid, one-step photoconversion of the poly(OEGMA) brush occurs that transforms it from a highly protein-resistant material to one that adsorbs protein and can covalently bind amine-containing molecules and that this photoconversion can be spatially addressed with high spatial resolution.