Type 3 iodothyronine deiodinase is selectively expressed in areas related to sexual differentiation in the newborn rat brain.
Type 3 iodothyronine deiodinase is selectively expressed in areas related to sexual differentiation in the newborn rat brain.
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DOI：
10.1210/en.140.11.5443
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发表时间：
1999-11-01
期刊：
影响因子：
4.8
通讯作者：
Bernal, J
中科院分区：
文献类型：
--
作者：
Escámez, MJ;Guadaño-Ferraz, A;Bernal, J
靶组织中甲状腺激素（T4和T3）浓度受碘甲状腺原氨酸脱碘酶活性的影响很大。1型和2型脱碘酶由T4生成T3，而3型脱碘酶（D3）将T4和T3转化为无活性代谢物。这些酶的表达和活性的协调被认为在生理和发育中起着重要作用，使个体细胞和组织根据特定需要调节活性激素的浓度成为可能。我们使用特异性35s标记的核糖探针原位杂交分析了D3在新生大鼠脑中的表达。在出生后0天，D3转录本被意外地发现选择性地表达在涉及大脑性别分化的区域，如终纹床核和视前核。这些区域的表达是短暂的，在出生后第10天不再观察到。这些观察结果表明，D3表达与决定性功能和行为的早期机制有关。
Thyroid hormone (T4 and T3) concentrations in target tissues are greatly influenced by the activity of iodothyronine deiodinases. Type 1 and 2 deiodinases generate T3 from T4, while deiodinase type 3 (D3) transforms T4 and T3 to inactive metabolites. Coordination of the expression and activity of these enzymes is postulated to play an important role in physiology and development, making it possible that individual cells and tissues regulate the concentrations of the active hormone according to specific needs. We have analyzed the expression of D3 in the neonatal rat brain by in situ hybridization using a specific 35S-labelled riboprobe. At postnatal day 0 D3 transcripts were unexpectedly found to be selectively expressed in areas involved in sexual differentiation of the brain such as the bed nucleus of the stria: terminalis and preoptic nuclei. Expression in these areas was transient and was no longer observed at postnatal day 10. These observations suggest that D3 expression is linked to the early mechanism determining sexual function and behavior.