Subtype-dependent postnatal development of direction- and orientation-selective retinal ganglion cells in mice
Subtype-dependent postnatal development of direction- and orientation-selective retinal ganglion cells in mice
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DOI：
10.1152/jn.00320.2014
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发表时间：
2014-11-01
影响因子：
2.5
通讯作者：
Tian, Ning
中科院分区：
文献类型：
--
作者：
Chen, Hui;Liu, Xiaorong;Tian, Ning
方向选择性神经节细胞（DSGCs）和取向选择性神经节细胞（OSGCs）分别编码运动物体的方向和取向信息。目前还不清楚DSGCs和OSGCs在出生后发育过程中如何在小鼠视网膜中成熟。在这里，我们调查了DSGCs和OSGCs的发展后，睁开眼睛。我们发现：1）DSGCs和OSGCs在出生后第12天（P12），就在睁眼前出现; 2）DSGCs和OSGCs的分数从P12到P30增加; 3）DSGCs和OSGCs的发育是亚型依赖性的; 4）方向和取向选择性是视网膜神经节细胞（RGC）在小鼠视网膜中的两个独立特征。我们根据RGCs的光反应特性将其分为不同的功能亚型。与P12相比，在P30，ON-OFF RGC的方向和取向选择性变得更强，而ON RGC没有。DSGCs的ON和ON-OFF两种亚型的调谐宽度均随年龄增长而减小。对于OSGCs，我们将其分为非方向选择性（non-DS）OSGCs和方向选择性OSGCs（DS&OSGCs）。对于DS&OSGCs，我们发现方向和取向选择性之间没有相关性，并且ON和ON-OFF亚型的调谐宽度随年龄保持不变。对于非DS OSGCs，ON而非ON-OFF亚型的调谐宽度随发育而减小。这些发现为揭示视网膜方向选择性发育的分子和突触机制提供了基础。
The direction-selective ganglion cells (DSGCs) and orientation-selective ganglion cells (OSGCs) encode the directional and the orientational information of a moving object, respectively. It is unclear how DSGCs and OSGCs mature in the mouse retina during postnatal development. Here we investigated the development of DSGCs and OSGCs after eye-opening. We show that 1) DSGCs and OSGCs are present at postnatal day 12 (P12), just before eye-opening; 2) the fractions of both DSGCs and OSGCs increase from P12 to P30; 3) the development of DSGCs and OSGCs is subtype dependent; and 4) direction and orientation selectivity are two separate features of retinal ganglion cells (RGCs) in the mouse retina. We classified RGCs into different functional subtypes based on their light response properties. Compared with P12, the direction and orientation selectivity of ON-OFF RGCs but not ON RGCs became stronger at P30. The tuning width of DSGCs for both ON and ON-OFF subtypes decreased with age. For OSGCs, we divided them into non-direction-selective (non-DS) OSGCs and direction-selective OSGCs (DS&OSGCs). For DS&OSGCs, we found that there was no correlation between the direction and orientation selectivity, and that the tuning width of both ON and ON-OFF subtypes remained unchanged with age. For non-DS OSGCs, the tuning width of ON but not ON-OFF subtype decreased with development. These findings provide a foundation to reveal the molecular and synaptic mechanisms underlying the development of the direction and orientation selectivity in the retina.