Efficacy of vaporized hydrogen peroxide against exotic animal viruses
Efficacy of vaporized hydrogen peroxide against exotic animal viruses
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DOI：
10.1128/aem.63.10.3916-3918.1997
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发表时间：
1997-10-01
影响因子：
4.4
通讯作者：
Sterritt, WG
中科院分区：
文献类型：
--
作者：
Heckert, RA;Best, M;Sterritt, WG
评价了直通箱中气相过氧化氢对可能被外来动物病毒污染的设备和无生命材料去污的有效性。进行了测试，vith各种病毒剂，其中包括几个病毒家族的代表（正粘病毒科、呼肠孤病毒科、黄病毒科、副粘病毒科、疱疹病毒科、小核糖核酸病毒科、杯状病毒科和弹状病毒科），特别强调加拿大外来的动物病毒。还研究了气体对各种实验室设备的影响。在玻璃和不锈钢上干燥卵液或血液，在暴露于气相过氧化氢30 min后评估病毒活力，对于所有测试的病毒和在所有条件下（除了一个），净化过程将病毒滴度降低到零，即禽流感病毒的胚胎致死剂量（禽流感和纽卡斯尔病病毒）或哺乳动物病毒少于10个组织培养感染剂量（非洲猪瘟、蓝舌病、猪瘟、伪狂犬病、猪水泡病、水泡性口炎和水泡性口炎病毒），暴露于气体的实验室设备似乎没有受到不利影响，气相过氧化氢去污可作为一种安全有效的方法，从处理外来动物疾病病毒剂的生物防护三级实验室中清除可能受病毒污染的物体。
The efficacy of vapor-phase hydrogen peroxide in a pass-through box for the decontamination of equipment and inanimate materials potentially contaminated with exotic animal viruses was evaluated. Tests were conducted,vith a variety of viral agents, which included representatives of several virus families (Orthomyxoviridae, Reoviridae, Flavivirinak, Paramyxoviridae, Herpesviridae, Picornaviridae, Caliciviridae, and Rhabdoviridae) from both avian and mammalian species, with particular emphasis on animal viruses exotic to Canada, The effects of the gas on a variety of laboratory equipment were also studied, Virus suspensions in cell culture media, egg fluid, or blood were dried onto glass and stainless steel, Virus viability was assessed after exposure to vapor-phase hydrogen peroxide for 30 min, For all viruses tested and under all conditions (except one), the decontamination process reduced the virus titer to 0 embryo-lethal doses for the avian viruses (avian influenza and Newcastle disease viruses) or less than 10 tissue culture infective doses for the mammalian viruses (African swine fever, bluetongue, hog cholera, pseudorabies, swine vesicular disease, vesicular exanthema, and vesicular stomatitis viruses), The laboratory equipment exposed to the gas appeared to suffer no adverse effects, Vapor-phase hydrogen peroxide decontamination can be recommended as a safe and efficacious way of removing potentially virus-contaminated objects from biocontainment level III laboratories in which exotic animal disease virus agents are handled.