Screening concepts for the isolation of biosurfactant producing microorganisms.
Screening concepts for the isolation of biosurfactant producing microorganisms.
复制标题
分离产生生物表面活性剂的微生物的筛选概念。
DOI：
10.1007/978-1-4419-5979-9_1
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发表时间：
2010
影响因子：
--
通讯作者：
R. Hausmann
中科院分区：
文献类型：
--
作者：
Vanessa Walter;C. Syldatk;R. Hausmann
本章概述了目前分离产生生物表面活性剂的微生物的方法。介绍了生物表面活性剂常用的筛选方法。细菌的取样和分离是筛选产生生物表面活性剂的微生物的基础。碳氢化合物污染的地点是分离产生生物表面活性剂的微生物最有希望的地方，但许多菌株也已从未受干扰的地点分离出来。在后续步骤中，必须对分离株进行表征，以鉴定对进一步研究感兴趣的菌株。已经开发了多种技术来鉴定产生生物表面活性剂的菌株。其中大多数直接基于培养物上清液的表面或界面活性。除此之外，一些筛选方法探索细胞表面的疏水性。这一特性也为生物表面活性剂的生产提供了指导。近年来，自动化和小型化导致了高通量筛选方法的发展。最后体现了用于分析大量潜在候选者或整个培养物收藏的高通量筛选（HTS）。然而，HTS 方法并没有引入新的原理。
This chapter gives an overview of current methods for the isolation of biosurfactant producing microbes. The common screening methods for biosurfactants are presented.Sampling and isolation of bacteria are the basis for screening of biosurfactant producing microbes. Hydrocarbon-contaminated sites are the most promising for the isolation of biosurfactant producing microbes, but many strains have also been isolated from undisturbed sites.In subsequent steps the isolates have to be characterized in order to identify the strains which are interesting for a further investigation. Several techniques have been developed for identifying biosurfactant producing strains. Most of them are directly based on the surface or interfacial activity of the culture supernatant. Apart from that, some screening methods explore the hydrophobicity of the cell surface. This trait also gives an indication on biosurfactant production.In recent years automation and miniaturization have led to the development of high throughput methods for screening. High throughput screening (HTS) for analyzing large amounts of potential candidates or whole culture collections is reflected in the end. However, no new principals have been introduced by HTS methods.