Advances in coupling droplet microfluidics to mass spectrometry.
Advances in coupling droplet microfluidics to mass spectrometry.
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DOI：
10.1016/j.copbio.2023.102962
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发表时间：
2023-06
影响因子：
7.7
通讯作者：
Bridget E. Murray;Laura I Penabad;R. T. Kennedy
中科院分区：
文献类型：
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作者：
Bridget E. Murray;Laura I Penabad;R. T. Kennedy
亮点Droplet-MS为低容量样品提供选择性的高通量分析。应用包括药物筛选、化学反应开发和体内检测。用于ESI和MALDI的Droplet联用方法能够分析不同的样品。Droplet微流体使开发低样品消耗和高通量的工作流程成为可能。基于荧光的分析大多用于液滴微流体；然而，对荧光报告器的要求限制了这种方法的适用性。液滴与质谱仪(MS)的耦合使得对复杂混合物的选择性分析成为可能，从而拓宽了分析范围。液滴微流体通过电喷雾电离(ESI)和基质辅助激光解吸电离(MALDI)被连接到MS。本文概述了这一新兴领域的发展，以及它在生物技术和生物分析中的应用的初步探索，包括合成生物学、反应发展和体内传感。
HighlightsDroplet-MS offers selective, high-throughput analysis for low volume samples.Applications include drug screening, chemical reaction development, and in vivo sensing.Droplet coupling methods for ESI and MALDI enable the analysis of diverse samples.Droplet microfluidics enables development of workflows with low sample consumption and high throughput. Fluorescence-based assays are most used with droplet microfluidics; however, the requirement of a fluorescent reporter restricts applicability of this approach. The coupling of droplets to mass spectrometry (MS) has enabled selective assays on complex mixtures to broaden the analyte scope. Droplet microfluidics has been interfaced to MS via electrospray ionization (ESI) and matrix-assisted laser desorption ionization (MALDI). The works reviewed herein outline the development of this nascent field as well as initial exploration of its application in biotechnology and bioanalysis, including synthetic biology, reaction development, and in vivo sensing.