Effect of streptozotocin-induced diabetes on myocardial blood flow reserve assessed by myocardial contrast echocardiography in rats.
Effect of streptozotocin-induced diabetes on myocardial blood flow reserve assessed by myocardial contrast echocardiography in rats.
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DOI：
10.1186/1475-2840-7-26
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发表时间：
2008-09-02
影响因子：
9.3
通讯作者：
Van Camp G
中科院分区：
文献类型：
--
作者：
Cosyns B;Droogmans S;Hernot S;Degaillier C;Garbar C;Weytjens C;Roosens B;Schoors D;Lahoutte T;Franken PR;Van Camp G
小血管结构和功能异常在糖尿病心肌病中的作用尚不清楚。心肌声学造影可以定量静息状态和潘生丁输注期间的心肌血流量。本研究的目的是用超声造影技术测定正常大鼠和链脲佐菌素诱导的糖尿病大鼠的心肌血流储备。我们前瞻性研究了40只Wistar大鼠。用链脲佐菌素诱发大鼠糖尿病模型20只。所有大鼠在麻醉基础和6个月后进行基线和应激（潘生丁：20 mg/kg）短轴视图的高功率间歇成像。心肌血流量测定和比较，在休息和潘生丁后，在这两个群体。计算并比较两组心肌血流储备。在研究结束时，从M型描记确定左心室功能参数，并对所有大鼠进行组织学检查。6个月时，糖尿病大鼠的心肌血流储备显著低于对照组（3.09 ± 0.98 vs. 1.28 ± 0.67 ml min-1 g-1; p < 0.05）。糖尿病组左心室功能明显下降，组织学上毛细血管表面积和直径明显减小。在这项动物研究中，糖尿病引起冠状动脉微循环的功能改变，如造影超声心动图所示，毛细血管密度和心脏收缩功能降低。这些发现可能为糖尿病心肌病的潜在机制提供新的见解。
The role of structural and functional abnormalities of small vessels in diabetes cardiomyopathy remains unclear. Myocardial contrast echocardiography allows the quantification of myocardial blood flow at rest and during dipyridamole infusion. The aim of the study was to determine the myocardial blood flow reserve in normal rats compared with Streptozotocin-induced diabetic rats using contrast echocardiography. We prospectively studied 40 Wistar rats. Diabetes was induced by intravenous streptozotocin in 20 rats. All rats underwent baseline and stress (dipyridamole: 20 mg/kg) high power intermittent imaging in short axis view under anaesthesia baseline and after six months. Myocardial blood flow was determined and compared at rest and after dipyridamole in both populations. The myocardial blood flow reserve was calculated and compared in the 2 groups. Parameters of left ventricular function were determined from the M-mode tracings and histological examination was performed in all rats at the end of the study. At six months, myocardial blood flow reserve was significantly lower in diabetic rats compared to controls (3.09 ± 0.98 vs. 1.28 ± 0.67 ml min-1 g-1; p < 0.05). There were also a significant decrease in left ventricular function and a decreased capillary surface area and diameter at histology in the diabetic group. In this animal study, diabetes induced a functional alteration of the coronary microcirculation, as demonstrated by contrast echocardiography, a decrease in capillary density and of the cardiac systolic function. These findings may offer new insights into the underlying mechanisms of diabetes cardiomyopathy.
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影响因子：
3.5
作者：
Bollano, Entela;Omerovic, Elmir;Fu, Michael
通讯作者：
Fu, Michael
影响因子：
7.7
作者：
JACKSON, CV;MCGRATH, GM;MCNEILL, JH
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MCNEILL, JH
影响因子：
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作者：
LITWIN, SE;KATZ, SE;DOUGLAS, PS
通讯作者：
DOUGLAS, PS
影响因子：
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作者：
NICHOLL, TA;LOPASCHUK, GD;MCNEILL, JH
通讯作者：
MCNEILL, JH
影响因子：
7.7
作者：
NITENBERG, A;VALENSI, P;ATTALI, JR
通讯作者：
ATTALI, JR