New hemocyte-specific enhancer-reporter transgenes for the analysis of hematopoiesis in Drosophila.
New hemocyte-specific enhancer-reporter transgenes for the analysis of hematopoiesis in Drosophila.
复制标题
用于分析果蝇造血作用的新血细胞特异性增强子报告基因转基因。
DOI：
10.1002/dvg.20561
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Schulz,RobertA
中科院分区：
文献类型：
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作者：
Tokusumi,Tsuyoshi;Shoue,DouglasA;Tokusumi,Yumiko;Stoller,JessicaR;Schulz,RobertA
Based on environmental challenges or altered genetic composition,Drosophilalarvae can produce up to three types of blood cells that express genetic programs essential for their distinct functions. Using transcriptional enhancers for genes expressed exclusively in plasmatocytes, crystal cells, or lamellocytes, several new hemocyte‐specific enhancer‐reporter transgenes were generated to facilitate the analysis ofDrosophilahematopoiesis. This approach took advantage of fluorescent variants of insulated P‐element reporter vectors for multilabeling cell analyses; two additional color variants were generated in these studies. These vectors were successfully used to produce transgenic fly lines that label specific hemocyte lineages with separate colors. Combining three transgene reporters allowed for the unambiguous identification of plasmatocytes, crystal cells, and lamellocytes within a complex hemocyte population. While this work focused on the hematopoietic process, these new vectors can be used to mark multiple cell types or trace complex cell lineages during any chosen aspect ofDrosophiladevelopment. genesis 47:771–774, 2009. © 2009 Wiley‐Liss, Inc.
