Carvacrol induces heat shock protein 60 and inhibits synthesis of flagellin in Eschefichia coli O157:H7v
Carvacrol induces heat shock protein 60 and inhibits synthesis of flagellin in Eschefichia coli O157:H7v
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DOI：
10.1128/aem.00340-07
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发表时间：
2007-07-01
影响因子：
4.4
通讯作者：
Veldhuizen, Edwin J. A.
中科院分区：
文献类型：
--
作者：
Burt, Sara A.;van der Zee, Ruurd;Veldhuizen, Edwin J. A.
牛至和百里香的精油对许多食源性病原体具有活性，如大肠杆菌O157：H7。香芹酚是这些油的主要抗菌成分之一，对伞花烃被认为是植物中的前体。研究了香芹酚和对伞花烃对大肠杆菌蛋白质合成的影响。coliO157：H7ATCC43895细胞。将细菌在具有亚致死浓度的香芹酚或对伞花烃的Mueller-Hinton肉汤中生长过夜，并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析其蛋白质组成，并通过Western印迹法确认。1 mM香芹酚过夜孵育引起E. coliO157：H7产生大量热休克蛋白60（GroEL）（P <0.05），极显著抑制鞭毛蛋白的合成（P <0.001），使细胞无鞭毛，不能运动。热休克蛋白70（DnaK）含量无明显变化。1 mM或10 mM的对伞花烃不诱导显著量的HSP 60或HSP 70，并且对鞭毛合成没有显著影响。香芹酚（0.3，0.5，0.8，或1 mM），也没有对伞花烃（0.3，0.5，或0.8 mM）治疗的细胞在中期指数生长期诱导显着量的HSP 60或HSP 70在3小时内，虽然观察到HSP 60的数值增加。运动性下降，这两种化合物的浓度增加，但现有的鞭毛没有脱落。这项研究首次证明了精油成分诱导细菌中的HSP 60，并且与香芹酚一起过夜孵育可以阻止E.大肠杆菌O157：H7。
The essential oils of oregano and thyme are active against a number of food-borne pathogens, such as Escherichia coli O157:H7. Carvacrol is one of the major antibacterial components of these oils, and p-cymene is thought to be its precursor in the plant. The effects of carvacrol and p-cymene on protein synthesis in E. coli O157:H7 ATCC 43895 cells were investigated. Bacteria were grown overnight in Mueller-Hinton broth with a sublethal concentration of carvacrol or p-cymene, and their protein compositions were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and confirmed by Western blotting. The presence of 1 mM carvacrol during overnight incubation caused E. coli O157:H7 to produce significant amounts of heat shock protein 60 (HSP60) (GroEL) (P < 0.05) and inhibited the synthesis of flagellin highly significantly (P < 0.001), causing cells to be aflagellate and therefore nonmotile. The amounts of HSP70 (DnaK) were not significantly affected. p-Cymene at 1 mM or 10 mM did not induce HSP60 or HSP70 in significant amounts and did not have a significant effect on flagellar synthesis. Neither carvacrol (0.3, 0.5, 0.8, or 1 mM) nor p-cymene (0.3, 0.5, or 0.8 mM) treatment of cells in the mid-exponential growth phase induced significant amounts of HSP60 or HSP70 within 3 h, although numerical increases of HSP60 were observed. Motility decreased with increasing concentrations of both compounds, but existing flagella were not shed. This study is the first to demonstrate that essential oil components induce HSP60 in bacteria and that overnight incubation with carvacrol prevents the development of flagella in E. coli O157:H7.