SEN1 gene from Lotus japonicus MG20 improves nitrogen fixation and plant growth
SEN1 gene from Lotus japonicus MG20 improves nitrogen fixation and plant growth
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DOI：
10.1080/00380768.2020.1834829
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发表时间：
2020-10
影响因子：
2
通讯作者：
Y. Nishida;Reona Hiraoka;Satomi Kawano;N. Suganuma;Shusei Sato;Satoshi Watanabe;T. Anai;S. Arima-S
中科院分区：
文献类型：
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作者：
Y. Nishida;Reona Hiraoka;Satomi Kawano;N. Suganuma;Shusei Sato;Satoshi Watanabe;T. Anai;S. Arima-S
摘要在百脉根中，固氮活性（乙炔还原活性，ARA）和种子重量的数量性状位点（QTL）位于4号染色体上与负责Fix−突变体sen 1的基因相同的区域。将L. Gifu B129与L. Miyakojima MG20，其导致一个氨基酸的缺失。在这里，我们研究了SEN1是否负责这些QTL。由于ARA和种子重量QTL的MG20等位基因具有正效应，我们产生了一个近等基因系（NIL），其中B129的SEN1基因区域被MG20所取代。接种中慢生根瘤菌Loti后21 d，该NIL（MG 20型）的生长参数显著高于B129型，ARA也显著高于B129型。与B129相比，突变体SEN1与MG20 SEN1基因互补的毛状根上形成的根瘤具有更高的ARA。MG20型近等基因系的种子显著重于B129型。这些结果表明，SEN1基因是一个非常可能的候选QTL的ARA和种子重量的第4染色体上。
ABSTRACT In Lotus japonicus, quantitative trait loci (QTLs) for nitrogen fixation activity (acetylene reduction activity, ARA) and seed weight are located in the same region on chromosome 4 as the gene responsible for the Fix− mutant sen1. The SEN1 gene of L. japonicus Gifu B129 has a deletion of three nucleotides in comparison with that of L. japonicus Miyakojima MG20, which results in a deletion of one amino acid. Here, we examined whether SEN1 is responsible for these QTLs. Because the MG20 allele of the QTL for ARA and seed weight has a positive effect, we generated a near-isogenic line (NIL) in which the SEN1 gene region of B129 was replaced with that of MG20. Growth parameters of this NIL (MG20-type) 21 days after inoculation with Mesorhizobium loti were significantly higher than those of B129-type, and ARA was also significantly higher. Nodules formed on hairy roots in which the sen1 mutant was complemented with the MG20 SEN1 gene had higher ARA than those of B129. The seeds of the MG20-type NIL were significantly heavier than those of B129-type. These results indicate that the SEN1 gene is a very likely candidate for the QTL for ARA and seed weight on chromosome 4.