Leishmania spp. parasite isolation through inoculation of patient biopsy macerates in interferon gamma knockout mice
Leishmania spp. parasite isolation through inoculation of patient biopsy macerates in interferon gamma knockout mice
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DOI：
10.1590/s0036-46652010000200004
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发表时间：
2010-04-01
期刊：
影响因子：
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通讯作者：
Ribeiro-Dias, Fátima
中科院分区：
文献类型：
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作者：
Oliveira, Milton Adriano Pelli de;Pires, Alause da Silva;Ribeiro-Dias, Fátima
Isolation of Leishmania parasite and species identification are important for confirmation and to help define the epidemiology of the leishmaniasis. Mice are often used to isolate pathogens, but the most common mouse strains are resistant to infection with parasites from the Leishmania (Viannia) subgenus. In this study we tested the inoculation of interferon gamma knockout (IFN gamma KO) mice with biopsy macerates from Leishmania-infected patients to increase the possibility of isolating parasites. Biopsies from twenty five patients with clinical signs of leishmaniasis were taken and tested for the presence of parasites. Immunohistochemical assay (IHC) and conventional histopathology detected the parasite in 88% and 83% of the patients, respectively. Leishmania sp. were isolated in biopsy macerates from 52% of the patients by culture in Grace's insect medium, but 13% of isolates were lost due to contamination. Inoculation of macerates in IFN gamma KO mice provides isolation of parasites in 31.8% of the biopsies. Most isolates belong to L. (Viannia) subgenus, as confirmed by PCR, except one that belongs to L. (Leishmania) subgenus. Our preliminary results support the use of IFN gamma KO mice to improve the possibility to isolate New World Leishmania species.
