Thy-1 variants of mouse lymphomas: biochemical characterization of the genetic defect
Thy-1 variants of mouse lymphomas: biochemical characterization of the genetic defect
复制标题
小鼠淋巴瘤的 Thy-1 变异：遗传缺陷的生化特征
DOI：
10.1016/0092-8674(75)90179-8
复制
发表时间：
1975
期刊：
影响因子：
64.5
通讯作者：
R. Hyman
中科院分区：
文献类型：
--
作者：
I. Trowbridge;R. Hyman
Thy-1阳性小鼠淋巴瘤和Thy-1阴性变体的表面蛋白通过乳过氧化物酶催化的碘化进行标记，并通过在十二烷基硫酸钠存在下的聚丙烯酰胺凝胶电泳和免疫学研究来表征。血清学上定义的Thy-1抗原决定簇的丧失与在Thy-1阴性变体的碘化蛋白质的放射自显影照片上对应于T淋巴细胞特异性表面糖蛋白T25的放射性带的缺失相关。 Thy-1在源自互补Thy-1阴性变体融合的杂交细胞上的重新表达与T25的再现相关。使用特异性识别T25的抗血清进行的定量吸收研究证实，Thy-1阴性变体在其表面上没有可检测到的T25（比Thy-1阳性系的T25低3倍）。生物合成标记研究表明，没有一个Thy-1阴性变体合成T25。然而，可以在两种变体中检测到免疫交叉反应分子的合成。基于这些结果，我们提出了一个模型，该模型描述了 Thy-1 抗原决定簇的可能结构，并解释了导致这些变体中 Thy-1 丢失的遗传损伤的生化性质。
The surface proteins of Thy-l positive mouse lymphomas and Thy-l negative variants were labeled by lactoperoxidase-catalyzed iodination and characterized by polyacrylamide gel electrophoresis in the presence of sodium dodecylsulphate and by immunological studies. The loss of the serologitally defined Thy-l antigenic determinant correlated with the absence of a radioactive band corresponding to a T lymphocyte-specific surface glycoprotein, T25, on autoradiographs of the iodinated proteins of Thy-l negative variants. Reexpression of Thy-l on hybrid cells derived from fusions of complementary Thy-l negative variants correlated with the reappearance of T25. Quantitative absorption studies using an antiserum which specifically recognized T25 confirmed that the Thy-l negative variants have no detectable T25 (to. 03 of that of the Thy-l positive lines) on their surface.Biosynthetic labeling studies revealed that none of the Thy-l negative variants synthesized T25. However, synthesis of an immunologically crossreactive molecule could be detected in two variants. On the basis of these results, we propose a model which describes a possible structure of the Thy-l antigenic determinant and explains the biochemical nature of the genetic lesions leading to the loss of Thy-l in these variants.